amyE
168

alpha-amylase

Locus

BSU_03040

Molecular weight

72.62 kDa

Isoelectric point

5.85

Protein length

660 aaSequenceBlast

Gene length

1980 bpSequenceBlast

Function

starch degradation

Product

alpha-amylase

Essential

E.C.

3.2.1.1

Synonyms

amyE, amyA

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0366 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

327,618 → 329,597

The protein

Catalyzed reaction/ biological activity

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units (according to UniProt)

Protein family

glycosyl hydrolase 13 family (according to UniProt)

Structure

1BAG (PDB) (complex with maltopentaose)

P00691 (AlphaFold)

Localization

secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Expression and Regulation

Operons

Genes

ycgB-amyE

Description

PubMed

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Genes

amyE

Description

PubMed

Regulation

repressed by glucose (CcpA) PubMed

Regulatory mechanism

CcpA: repression, PubMed, in ccpA regulon

AbrB: activation, PubMed, in abrB regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

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Biological materials

Mutant

GP550 (cat), available in Stülke lab

BKE03040 (ΔamyE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGG

BKK03040 (ΔamyE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTGACACTCCTTATT, downstream forward: _UP4_TGAGGGCAAGGCTAGACGGG

References

Reviews

Original Publications

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Time of last update: 2025-04-07 04:22:30

Author of last update: Melvin.boenninger

amyE 168

amyE
168