comFA

comFA
168

membrane-associated DNA-dependent ATPase, may provide energy for DNA transport

Locus
BSU_35470
Molecular weight
52.40 kDa
Isoelectric point
9.82
Protein length
463 aaSequenceBlast
Gene length
1392 bpSequenceBlast
Function
genetic competence
Product
ATP-binding protein
Essential
no
Synonyms
comFA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4098 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,642,167  3,643,558
Phenotypes of a mutant
knockout has 1000-fold reduction in transformability compared to the wild-type PubMed
The protein
Catalyzed reaction/ biological activity
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
helicase family (according to UniProt)
Domains
Helicase ATP-binding domain (aa 133-285) (according to UniProt)
Helicase C-terminal domain (aa 317-463) (according to UniProt)
Cofactors
Zn2+ PubMed
Structure
P39145 (AlphaFold)
Modification
phosphorylated on Arg-20, Arg-186, and Arg-446 PubMed
Localization
cell membrane PubMed
localizes to cell poles in competent cells PubMed
localizes to cell poles PubMed
localizes along the long axis of competent cells PubMed
Expression and Regulation
Operons
Genes
comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL-yviE-fliW-csrA
Description
PubMed
Expression
expression is heterogeneous PubMed
Regulatory mechanism
ComK: activation, PubMed, in comK regulon
DegU: activation, DegU-P, PubMed, in degU regulon
ScoC: repression, PubMed, in scoC regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigD: sigma factor, PubMed, in sigD regulon
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comFA→csrA

2025-03-28 01:21:10

Jstuelk

156

F13A7084BCCB15345BC26C03AF15EE753EFDF196

11180672E46D33F7D747E58441ECB10AEE7F8D8B

Biological materials
Mutant
BKE35470 (comFA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATAGCACGCCTCCTTTC,  downstream forward: _UP4_TAGCCATTATTTGAAAACGT
BKK35470 (comFA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACATAGCACGCCTCCTTTC,  downstream forward: _UP4_TAGCCATTATTTGAAAACGT
References
Reviews
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Original Publications
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45F27C0789199626BA90D2C69E8F13525C911478

Page visits: 4992

Time of last update: 2025-04-01 22:58:16

Author of last update: Jstuelk