glsA

glsA
168

glutaminase, low affinity for glutamine

Locus
BSU_02430
Molecular weight
36.03 kDa
Isoelectric point
5.32
Protein length
327 aaSequenceBlast
Gene length
984 bpSequenceBlast
Function
glutamine degradation
Product
glutaminase
Essential
no
E.C.
3.5.1.2
Synonyms
glsA, ybgJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2066 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
264,191  265,174
The protein
Catalyzed reaction/ biological activity
H2O + L-glutamine --> L-glutamate + NH4+ (according to UniProt)
Protein family
glutaminase family (with YlaM, according to UniProt)
Structure
3BRM (PDB) 1MKI (PDB)PubMed
O31465 (AlphaFold)
Paralogous protein(s)
YlaM
Expression and Regulation
Operons
Genes
glsA-glnT
Description
PubMed
Regulation
induced in the presence of glutamine (GlnL) PubMed
Regulatory mechanism
GlnL: activation, PubMed, in glnL regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

glsA→glnT

2025-04-04 07:59:51

ghost

77

1abc9708789b9480434727fa47a0c483a7d39864

AA0FCFADA92CADCC16FD2BF6A3187BADD37324D7

Biological materials
Mutant
GP4253 (ΔglsA::cat), available in Jörg Stülke's lab
MGNA-B943 (ybgJ::erm), available at the NBRP B. subtilis, Japan
BKE02430 (glsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAATTCCTCCTCACTG,  downstream forward: _UP4_TAAACATGAAAAAAGAGGTG
BKK02430 (glsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGAATTCCTCCTCACTG,  downstream forward: _UP4_TAAACATGAAAAAAGAGGTG
References
Loading

1A415DC85354373EA4866733C3AE43F510BD3C56

Page visits: 3628

Time of last update: 2025-04-05 01:10:47

Author of last update: Jstuelk