holB

holB
168

DNA polymerase III (delta subunit), part of the clamp-loader complex and the replisome

Locus
BSU_00310
Molecular weight
37.47 kDa
Isoelectric point
6.33
Protein length
329 aaSequenceBlast
Gene length
990 bpSequenceBlast
Function
DNA replication
Product
DNA polymerase III (delta subunit)
Essential
yes
E.C.
2.7.7.7
Synonyms
holB, yaaS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0470 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
40,665 → 41,654
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
required for bacteriophage SPP1 replication PubMed
Structure
1NJG (PDB) (from E. coli, 36% identity) PubMed
P37540 (AlphaFold)
Localization
forms foci PubMed
cytoplasm (homogeneous) PubMed
Expression and Regulation
Operons
Genes
xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC
Description
PubMed
Open in new tab

xpaC→yabC

2025-03-25 19:55:04

Jstuelk

196

adcd0181c3bf7f5a66d1644d176d0c60f2121a3a

87CEB0D67D1455F48A42F665A0B9A91B779AEC5D

Genes
darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC
Description
PubMed
Regulation
expressed during growth and the transition phase, expression is erduced in stationary phase PubMed
Open in new tab

darA→yabC

2025-04-05 02:05:55

ghost

180

62a125e6301e56c82f6a74dcb70741fe56737f41

DA0A2CE2876D38C223E4BA512D01D105B53BF136

Genes
tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC
Description
PubMed
Open in new tab

tmk→yabC

2025-04-05 08:43:18

Jstuelk

183

cae86a5ed186d19f5321f28f72d35514a5988afb

E9A27179465E2479F141CE804267881CAB860CC2

Biological materials
Expression vectors
pGP1692: expression of Strep-holB-Strep in E. coli, in pGP172, available in Jörg Stülke's lab
pGP1698: expression of holB-Strep in E. coli, in pGP574, available in Jörg Stülke's lab
pGP1699: expression of holB in E. coli, in pGP574 (no tag due to a stop codon at the end of holB), available in Jörg Stülke's lab
pGP2672: expression of Strep-holB by pGP380 in B. subtilis, suitable for SPINE, available in Jörg Stülke's lab
pGP3028: expression of holB-Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
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DC867376EF4F9CC7663A9B76AA260AC3CA11595A

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Time of last update: 2025-04-04 12:46:04

Author of last update: Jstuelk