lplJ
168

lipoate-protein ligase

Locus

BSU_10250

Molecular weight

37.86 kDa

Isoelectric point

5.84

Protein length

331 aaSequenceBlast

Gene length

996 bpSequenceBlast

Function

scavenging of lipoic acid

Product

Lipoate:protein ligase

Essential

Synonyms

lplJ, yhfJ

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0095 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

1,099,159 → 1,100,154

The protein

Catalyzed reaction/ biological activity

attaches exogenous lipoic acid to the apoproteins by a two-step ATP dependent reaction:

a) the activation of lipoic acid to lipoyl-AMP PubMed

b) the transfer of this activated lipoyl species to E2 subunit of 2-oxoglutarate dehydrogenase (OdhB) and GcvH, with the concomitant liberation of AMP PubMed

(R)-lipoate + [lipoyl-carrier protein]-L-lysine + ATP --> [lipoyl-carrier protein]-(R)-N⁶-lipoyl-L-lysine + AMP + diphosphate + H⁺ (according to UniProt)

Protein family

LplA family (single member, according to UniProt)

Domains

BPL/LPL catalytic domain (aa 27-214) (according to UniProt)

Structure

5IBY (PDB) (from Enterococcus faecalis, 59% identity)

O07608 (AlphaFold)

Expression and Regulation

Operons

Genes

yhfI-lplJ-yhfK

Description