narI

narI
168

nitrate reductase (gamma subunit)

Locus
BSU_37250
Molecular weight
25.15 kDa
Isoelectric point
10.04
Protein length
223 aaSequenceBlast
Gene length
672 bpSequenceBlast
Function
nitrate respiration, nitrogen assimilation
Product
nitrate reductase (gamma subunit)
Essential
no
Synonyms
narI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2181 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,823,558  3,824,229
The protein
Catalyzed reaction/ biological activity
quinol + nitrate --> quinone + H2O + nitrite (according to UniProt)
Cofactors
heme b PubMed
Structure
1Q16 (PDB) (the NarG-NarH-NarI complex of E. coli, 37% identity) PubMed
P42177 (AlphaFold)
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
narG-narH-narJ-narI
Description
PubMed
Regulation
expressed under anaerobic conditions (Fnr) PubMed
Regulatory mechanism
Fnr: activation, PubMed, in fnr regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Open in new tab

narG→narI

2025-04-04 05:20:34

ghost

94

2df0c9c15bd640f1035afac39ab71391c14fde4b

655A38484A21CE64690D3913F055D0C46476DC9F

Biological materials
Mutant
BKE37250 (narI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATACCCCAGAGGATCTGCC,  downstream forward: _UP4_TGAAAATCCCCCTTGCAAGA
BKK37250 (narI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATACCCCAGAGGATCTGCC,  downstream forward: _UP4_TGAAAATCCCCCTTGCAAGA
References
Reviews
Loading
Original Publications
Loading

20060F4C1939FB9A10CB279745D75D0A16627EC6

Page visits: 2636

Time of last update: 2025-04-03 14:44:57

Author of last update: Jstuelk