ndk

ndk
168

nucleoside diphosphate kinase

Locus
BSU_22730
Molecular weight
16.82 kDa
Isoelectric point
5.57
Protein length
149 aaSequenceBlast
Gene length
450 bpSequenceBlast
Function
maintainance of an equilibrium between the concentrations of different nucleoside triphosphates
Product
nucleoside diphosphate kinase
Essential
no
E.C.
2.7.4.6
Synonyms
ndk
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0105 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,381,354 → 2,381,803
Phenotypes of a mutant
a ndk pyk double mutant is unable to grow on minimal medium with glucose due to lack of NTP production PubMed
inactivation of ''ndk'' reduces sporulation efficiency to 13.4% that of wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
2'-deoxyribonucleoside 5'-diphosphate + ATP --> 2'-deoxyribonucleoside 5'-triphosphate + ADP (according to UniProt)
ribonucleoside 5'-diphosphate + ATP --> ribonucleoside 5'-triphosphate + ADP (according to UniProt)
Protein family
NDK family (single member, according to UniProt)
Structure
1WKJ (PDB) (from Thermus thermophilus, 59% identity, 82% similarity)
P31103 (AlphaFold)
Modification
phosphorylation on Thr-92 AND Ser-123 PubMed
Expression and Regulation
Operons
Genes
hepS-menH-hepT-ndk
Description
PubMed
Open in new tab

hepS→ndk

2025-04-03 22:19:21

Jstuelk

141

4994bd02adb7701a42b6eebb6e0352c9f8a44e38

8B6317CB1523DAD0B462E932EA20388911064C0F

Genes
ndk
Description
PubMed
Open in new tab

ndk

2025-04-05 07:18:35

Jstuelk

88

e78049fbd9f94bf70690facd3b3da013daab20cb

D0313F4563B02CC62065820FCC46E3BC389E3D38

Additional information
expression is reduced in motile cells as compared to non-motile cells PubMed
Biological materials
Mutant
BKE22730 (Δndk::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCTCCACCCCATCAT,  downstream forward: _UP4_TAATCATTGTGAAAATAAGC
BKK22730 (Δndk::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCTCCACCCCATCAT,  downstream forward: _UP4_TAATCATTGTGAAAATAAGC
References
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B498ED03F19B7A73878D5C947C28C2A87CAB934D

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Time of last update: 2025-04-05 01:35:33

Author of last update: Jstuelk