nsrR

nsrR
168

nitric oxide-responsive regulator

Locus
BSU_09380
Molecular weight
16.47 kDa
Isoelectric point
7.75
Protein length
146 aaSequenceBlast
Gene length
441 bpSequenceBlast
Function
repression of ResD-ResE-dependent genes in the absence of nitric oxide (NO)
Product
transcriptional repressor
Essential
no
Synonyms
nsrR, yhdE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1959 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,013,411 → 1,013,851
The protein
Domains
HTH rrf2-type domain (aa 2-133) (according to UniProt)
Cofactors
Fe-S cluster, required for DNA-binding PubMed
Structure
3LWF (PDB) (from Listeria innocua, 29% identity)
O07573 (AlphaFold)
Effectors of protein activity
nitric oxide (NO) acts as molecular inducer and results in NsrR release from its DNA targets PubMed
[4Fe-4S]-NsrR binds around the -35 element of the ''nasD'' promoter with much higher affinity than apo-NsrR and binding of [4Fe-4S]-NsrR, but not apo-protein, is sensitive to NO PubMed
Expression and Regulation
Operons
Genes
nsrR
Description
PubMed
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nsrR

2025-03-15 08:48:20

ghost

77

58cb32932ccc2aa1f81b958f9b6401d271832210

0733B43DF27AF8F1C9372D0E3F4C488F0E404830

Biological materials
Mutant
MGNA-A692 (yhdE::erm), available at the NBRP B. subtilis, Japan
BKE09380 (ΔnsrR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATAAGACCTCAGAATA,  downstream forward: _UP4_TAGATTAAGATTCCTTCTTT
BKK09380 (ΔnsrR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATAAGACCTCAGAATA,  downstream forward: _UP4_TAGATTAAGATTCCTTCTTT
References
Reviews
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Other original publications
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The NsrR regulon
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EC6697D5D945B7E5083AFED9218748763C443278

Page visits: 3887

Time of last update: 2025-04-05 14:43:28

Author of last update: Melvin.boenninger