obg
168

GTP-binding protein involved in initiation of sporulation and assembly of the 50S ribosome subunit

Locus

BSU_27920

Molecular weight

47.53 kDa

Isoelectric point

4.86

Protein length

428 aaSequenceBlast

Gene length

1287 bpSequenceBlast

Function

ribosome assembly, may be required to stimulate activity

Product

GTP-binding protein

Essential

Yes

E.C.

3.6.5.-

Synonyms

obg

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0536 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,852,661 2,853,947

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

binds and hydrolyzes GTP and readily exchanges GDP for GTP PubMed

binds to the premature 50S ribosomal subunit and facilitates recruitment of rproteins and rRNA processing to form the mature 50S subunit PubMed

binds GDP PubMed

binds ppGpp PubMed

binds GTP + Mg(2+) PubMed

Protein family

TRAFAC class OBG-HflX-like GTPase superfamily (with YnbA and YyaF, according to UniProt)

Domains

Obg possesses 3 domains: A N-terminal glycine-rich domain (1158), a GTP binding domain (159342) and a C-terminal TGS domain (343428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.

Obg domain (aa 1-158) (according to UniProt)

OBG-type G domain (aa 159-329) (according to UniProt)

OCT domain (aa 350-428) (according to UniProt)

Cofactors

GDP, GTP and probably ppGpp

Structure

1LNZ (PDB) PubMed

P20964 (AlphaFold)

Paralogous protein(s)

YqeH, YnbA, EngA, YyaF, Era, YsxC, RbgA, ThdF

Localization

cytoplasm, throughout the cell PubMed

Additional information

The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in B. subtilis.

Expression and Regulation

Operons

Genes

spo0B-obg-thrR-pheA

Description

PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Additional information

expression is repressed by binding of RpoE to the A-rich sequence in the -35 region of the promoter PubMed

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Biological materials