pbpH

pbpH
168

class B penicillin-binding protein H, required for cell wall synthesis during cell elongation, works together with RodA in the elongasome for lateral cell wall synthesis and control of cell diameter

Locus
BSU_13980
Molecular weight
76.98 kDa
Isoelectric point
8.89
Protein length
Gene length
Function
formation of a rod-shaped peptidoglycan cell wall
Product
penicillin-binding protein H
Essential
no
Synonyms
pbpH, ykuA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,467,805  1,469,862
Phenotypes of a mutant
a pbpA pbpH double mutant is not viable PubMed
The protein
Catalyzed reaction/ biological activity
required for cell wall synthesis during cell elongation PubMed
driver of MreB dynamics PubMed
Protein family
transpeptidase family (according to UniProt)
Structure
2WAF (PDB) (Pbp2B from Streptococcus pneumoniae, 31% identity) PubMed
Paralogous protein(s)
during vegetative growth: evenly distributed along the membrane PubMed
localization depends on the availability of peptidoglyan precursors (lipid II) PubMed
Additional information
loss of activity at alkaline pH8.5 PubMed
Expression and Regulation
Operons
Genes
Description
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
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pbpH

2025-03-16 16:15:21

Bzhu

107

c990d00eaa2db6e7b0c5cbddef8bf6be7dbe42b0

D867CA47C299E56CF6EF1C5B01367525783C47C4

Biological materials
Mutant
MGNA-B335 (ykuA::erm), available at the NBRP B. subtilis, Japan
BKE13980 (pbpH::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC,  downstream forward: _UP4_TAAAAAACAGGGTGCACAAC
BKK13980 (pbpH::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATTCTGATTGCTCTGTTTC,  downstream forward: _UP4_TAAAAAACAGGGTGCACAAC
GFP fusion
3140 trpC2 pbpH::pSG5058 (cat Pxyl-gfpa-pbpH1-827) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC
References
Reviews
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Original Publications
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Time of last update: 2025-04-07 02:08:21

Author of last update: Jstuelk