pyrAA

pyrAA
168

carbamoyl-phosphate synthetase (glutaminase subunit)

Locus
BSU_15510
Molecular weight
39.96 kDa
Isoelectric point
5.82
Protein length
364 aaSequenceBlast
Gene length
1095 bpSequenceBlast
Function
pyrimidine biosynthesis
Product
carbamoyl-phosphate synthetase (glutaminase subunit)
Essential
no
E.C.
6.3.5.5
Synonyms
pyrAA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0505 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,622,657 → 1,623,751
The protein
Catalyzed reaction/ biological activity
2 ATP + H2O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphate (according to UniProt)
Protein family
CarA family (with CarA, according to UniProt)
Domains
Glutamine amidotransferase type-1 domain (aa 171-356) (according to UniProt)
Structure
1JDB (PDB) (from E. coli, 47% identity) PubMed
P25993 (AlphaFold)
Paralogous protein(s)
CarA
Expression and Regulation
Operons
Genes
pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
Description
PubMed
Regulation
induced in the absence of uridine nucleotides (PyrR) PubMed
Regulatory mechanism
PyrR: termination, via RNA switch, in pyrR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pyrR→pyrE

2025-03-26 16:59:11

ghost

111

3b978222b0b6a5230a2e7701ac35d1e35cc85307

0A8FD5CB6489EDD8CB2074130B763DCAC1F24654

Biological materials
Mutant
BKE15510 (ΔpyrAA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC,  downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC
BKK15510 (ΔpyrAA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTTTCCAGTACTAATCGTC,  downstream forward: _UP4_ACAGAGAAAGAAGGGGAAGC
GP3716 (pyrAA::aphA3 trpC2) available in the Stülke lab
GP3719 (pyrAAAB::aphA3 trpC2) available in the Stülke lab
GP3721 (pyrAAAB::cat trpC2) available in the Stülke lab
Expression vectors
pGP3772: expression of Strep-pyrAA by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
References
Reviews
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Original Publications
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E7741ED6F15044900BDC5F0584246469D2D4D586

Page visits: 3198

Time of last update: 2025-04-05 15:43:31

Author of last update: Robert.warneke