pyrC

pyrC
168

dihydroorotase

Locus
BSU_15500
Molecular weight
46.45 kDa
Isoelectric point
5.43
Protein length
428 aaSequenceBlast
Gene length
1287 bpSequenceBlast
Function
pyrimidine biosynthesis
Product
dihydroorotase
Essential
no
E.C.
3.5.2.3
Synonyms
pyrC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0044 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, PyrR regulon

Gene
Coordinates
1,621,374 → 1,622,660
The protein
Catalyzed reaction/ biological activity
(S)-dihydroorotate + H2O --> H+ + N-carbamoyl-L-aspartate (according to UniProt)
Protein family
Metallo-dependent hydrolases superfamily (according to UniProt)
Structure
3GRI (PDB) (from Staphylococcus aureus subsp. aureus mw2, 60% identity, 72% similarity)
P25995 (AlphaFold)
Paralogous protein(s)
PucH
Expression and Regulation
Operons
Genes
pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
Description
PubMed
Regulation
induced in the absence of uridine nucleotides (PyrR) PubMed
Regulatory mechanism
PyrR: termination, via RNA switch, in pyrR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pyrR→pyrE

2025-03-26 16:59:11

ghost

111

3b978222b0b6a5230a2e7701ac35d1e35cc85307

0A8FD5CB6489EDD8CB2074130B763DCAC1F24654

Biological materials
Mutant
BKE15500 (ΔpyrC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC,  downstream forward: _UP4_TATGAAGAGGGGAGACTTGT
BKK15500 (ΔpyrC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTTTCGTTTAGTATCCAGC,  downstream forward: _UP4_TATGAAGAGGGGAGACTTGT
References
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Time of last update: 2025-04-04 22:58:15

Author of last update: Jstuelk