rodZ

rodZ
168

required for proper septum positioning during vegetative growth (cell shape determination), part of the Rod complex for lateral cell wall synthesis and control of cell diameter

Locus
BSU_16910
Molecular weight
23.48 kDa
Isoelectric point
4.68
Protein length
288 aaSequenceBlast
Gene length
867 bpSequenceBlast
Function
septum positioning during vegetative growth
Product
morphogenic protein
Essential
yes
Synonyms
rodZ, ymfM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1426 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,761,707  1,762,573
Phenotypes of a mutant
essential PubMed
depletion results in the formation of shorter and rounder cells PubMed
depletion results in disturbed formation of asymmetric septa and perturbed SigF activation PubMed
hypersensitivity to sorbic acid stress PubMed
small, round, DNA-containing cells PubMed
the mutant cells are thicker and longer than wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
the presence of RodZ-P results in increased MreB filament density and growth rate PubMed
Domains
N-terminal cytoplasmic domain (RodZn), a transmembrane domain, and C-terminal extra-cytoplasmic domain (RodZc) PubMed
Structure
3FYM (PDB) (from Staphylococcus aureus, corresponds to aa 1 ... 112, 31% identity)
O31771 (AlphaFold)
Modification
phosphorylation by PrkC in response to lipid II abundance PubMed
Localization
membrane protein (according to UniProt)
Additional information
overexpression results in growth inhibition in minimal medium (in the absence of Mg2+ ) PubMed
RodZ is destabilized in a ''mreB'' mutant PubMed
Expression and Regulation
Operons
Genes
ymfK/1-ymfK/2-rodZ
Description
PubMed
Open in new tab

ymfK/1→rodZ

2025-03-22 10:41:17

Jstuelk

104

b950e91cac4165ef381d23ef9a8e7e69ea47bc84

11B94A37223A14387217043C4CD3317EAFFE5485

Biological materials
Mutant
MGNA-B120 (ymfM::erm), available at the NBRP B. subtilis, Japan
BKE16910 (rodZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG,  downstream forward: _UP4_TAATTACCAGATGACTTTTC
BKK16910 (rodZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG,  downstream forward: _UP4_TAATTACCAGATGACTTTTC
Labs working on this gene/protein
Adriano Henriques, Lisbon, Portugal  homepage
References
Reviews
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Original Publications
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6071A84EF190C820DB8985C08ABC74F744B793DB

Page visits: 6906

Time of last update: 2025-04-05 04:25:55

Author of last update: Jstuelk