sacT
168

transcriptional antiterminator for the sacP-sacA-ywdA operon

Locus

BSU_38070

Molecular weight

31.92 kDa

Isoelectric point

5.59

Protein length

276 aaSequenceBlast

Gene length

831 bpSequenceBlast

Function

regulation of sucrose utilization

Product

transcriptional antiterminator

Essential

Synonyms

sacT, ipa-47d

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3711 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,906,142 3,906,972

The protein

Catalyzed reaction/ biological activity

binding to the mRNA of the ''sacP-sacA'' operon, causes transcription antitermination (in presence of sucrose and absence of glucose)

Protein family

PRD-containing transcription factors

Domains

N-terminal RNA binding domain PubMed

2 x PRD (PtsI regulation domains) PubMed

2 PRD domains (aa 62-167, aa 168-276) (according to UniProt)

Structure

1TLV (PDB) (the PRD domains of LicT, corresponds to aa 54 ... 271, 41% identity) PubMed

P26212 (AlphaFold)

Modification

Phosphorylated and inactivated by SacP (EII^Scr) (according to Swiss-Prot)

Paralogous protein(s)

GlcT, SacY, LicT

Expression and Regulation

Operons

Genes

sacT

Description

PubMed

Regulation

repressed by casamino acids PubMed

Regulatory mechanism

DnaA: repression, PubMed, in dnaA regulon

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Genes

ywcI-sacT

Description

PubMed

Regulation

repressed by casamino acids PubMed

Regulatory mechanism

DnaA: repression, PubMed, in dnaA regulon

Additional information

the mRNA is substantially stabilized upon depletion of RNase Y PubMed

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Biological materials

Mutant

GP429 (spc), available in Jörg Stülke's lab

BKE38070 (sacT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAGGTTATCTCTCCCGC, downstream forward: _UP4_TAACCGCTTTGACTTGCAGG

BKK38070 (sacT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGAGGTTATCTCTCCCGC, downstream forward: _UP4_TAACCGCTTTGACTTGCAGG

Expression vectors

for expression, purification of both PRDs in E. coli with N-terminal His-tag, in pWH844: pGP166, available in Jörg Stülke's lab

for expression, purification of PRD-1 in E. coli with N-terminal His-tag, in pWH844: pGP426, available in Jörg Stülke's lab

for expression, purification of PRD-2 in E. coli with N-terminal His-tag, in pWH844: pGP427, available in Jörg Stülke's lab

for expression, purification of PRD-1 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP439, available in Jörg Stülke's lab

for expression, purification of PRD-2 in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP440, available in Jörg Stülke's lab

for expression, purification of RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP571, available in Jörg Stülke's lab

for expression of RNA-binding domain in B. subtilis, in pBQ200: pGP446, available in Jörg Stülke's lab

for expression, purification of sacT-full length in B. subtilis with C-terminal Strep-tag, in pGP382: pGP1064, available in Jörg Stülke's lab

for expression, purification of sacT-full length in B. subtilis with N-terminal Strep-tag, in pGP380: pGP1068, available in Jörg Stülke's lab

FLAG-tag construct

GP1223 (spc, based on pGP1331), available in Jörg Stülke's lab

GFP fusion

GP1227 (spc, based on pGP1870), available in Jörg Stülke's lab

References

Reviews

Original Publications

Page visits: 4140

Time of last update: 2025-04-04 11:37:55

Author of last update: Melvin.boenninger

sacT 168

sacT
168