sipW

sipW
168

bifunctional signal peptidase I that controls surface-adhered biofilm formation and processes TasA and TapA

Locus
BSU_24630
Molecular weight
20.53 kDa
Isoelectric point
5.49
Protein length
190 aaSequenceBlast
Gene length
573 bpSequenceBlast
Function
biofilm formation
Product
signal peptidase I
Essential
no
Synonyms
sipW, yqhE
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0681 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,553,930  2,554,502
The protein
Catalyzed reaction/ biological activity
important for biofilm formation on a solid surface, but not required at an air-liquid interface PubMed
Cleavage of hydrophobic, N-terminal signal or leader sequences from TasA and TapA PubMed
Protein family
peptidase S26B family (single member, according to UniProt)
Structure
P54506 (AlphaFold)
Localization
membrane PubMed
Expression and Regulation
Operons
Genes
tapA-sipW-tasA
Description
PubMed
Regulation
repressed by casamino acids PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
SinR: repression, PubMed, in sinR regulon
RemA: activation, PubMed, in remA regulon
LutR: activation, PubMed, in lutR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
expression of the operon is localized to a ring near the periphery of the biofilm PubMed
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tapA→tasA

2025-04-05 17:22:14

Jstuelk

153

5d4b2839ae781570552ba6a28b48e7489d628dd2

25F0FAE210D5101B1935E4625742633C173FC509

Biological materials
Mutant
MGNA-C439 (yqhE::erm), available at the NBRP B. subtilis, Japan
1A937 ( sipW::kan), PubMed, available at BGSC
BKE24630 (sipW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGTAAAGATGATCACGTATA,  downstream forward: _UP4_TAACTTCAGTTGTAAACCTG
BKK24630 (sipW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGTAAAGATGATCACGTATA,  downstream forward: _UP4_TAACTTCAGTTGTAAACCTG
Labs working on this gene/protein
Beth Lazazzera, Los Angeles, USA
References
Reviews
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Original Publications
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09B082BF39703F0D9A5980E643175DBD59F5B228

Page visits: 5529

Time of last update: 2025-04-06 08:47:23

Author of last update: Jstuelk