trmFO

trmFO
168

flavoprotein, tRNA:m(5)U-54 methyltransferase, glucose-inhibited division protein

Locus
BSU_16130
Molecular weight
47.90 kDa
Isoelectric point
5.77
Protein length
435 aaSequenceBlast
Gene length
1308 bpSequenceBlast
Function
tRNA modification
Product
tRNA:m(5)U-54 methyltransferase
Essential
no
E.C.
2.1.1.74
Synonyms
trmFO, ylyC, gid
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1206 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,685,812 → 1,687,119
The protein
Catalyzed reaction/ biological activity
catalyzes the folate-dependent C(5)-methylation of uridine at position 54 in the TpsiC loop of tRNA
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + FADH2 + uridine54 in tRNA --> (6S)-5,6,7,8-tetrahydrofolate + 5-methyluridine54 in tRNA + FAD + H+ (according to UniProt)
Protein family
MnmG family (with GidA, according to UniProt)
Cofactors
FAD, methylene-THF PubMed
Structure
3G5S (PDB) (TrmFO from Thermus thermophilus, 54% identity, 79% similarity) PubMed
P39815 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
trmFO-codV-clpQ-clpY-codY
Description
PubMed
Regulation
repressed during growth in the presence of branched chain amino acids (CodY) PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the intracellular concentration of CodY is about 2.5 myM (according to PubMed)
Open in new tab

trmFO→codY

2025-04-03 22:05:52

Jstuelk

126

14a27ac38158bfce7849c72f786e8673bc2ce6b1

5257B6B5AFCAE25607CF5DD2E50F759EF0225538

Biological materials
Mutant
MGNA-B147 (gid::erm), available at the NBRP B. subtilis, Japan
BKE16130 (ΔtrmFO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTCACATCTCCTAGT,  downstream forward: _UP4_TAGGTATTGATTGCAACCTG
BKK16130 (ΔtrmFO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTCACATCTCCTAGT,  downstream forward: _UP4_TAGGTATTGATTGCAACCTG
References
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B6062575849EA65BB7B44A9723676885799E2759

Page visits: 4893

Time of last update: 2025-04-03 22:50:31

Author of last update: Jstuelk