prkD
168
protein kinase D
Locus
BSU_02030
Molecular weight
29.91 kDa
Isoelectric point
6.36
Function
unknown
Product
protein kinase D
Essential
no
E.C.
2.7.11.1
Synonyms
prkD, ybdM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0515 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
223,219 → 223,989
The protein
Catalyzed reaction/ biological activity
ATP + L-seryl-[protein] --> ADP + H+ + O-phospho-L-seryl-[protein] (according to UniProt)
ATP + L-threonyl-[protein] --> ADP + H+ + O-phospho-L-threonyl-[protein] (according to UniProt)
Protein family
protein kinase superfamily (according to UniProt)
Ser/Thr protein kinase family (according to UniProt)
Protein kinase domain (aa 25-256) (according to UniProt)
Structure
4EQM (PDB) (PknB from Staphylococcus aureus, 34% identity in the N-terminal part, aa 18 ... 174) PubMed
Modification
autophosphorylated on Thr-174 (in vitro) PubMed
Biological materials
Mutant
MGNA-B958 (ybdM::erm), available at the NBRP B. subtilis, Japan
GP578 (aphA3), available in Jörg Stülke's lab
Expression vectors
pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Jörg Stülke's lab, PubMed
for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Jörg Stülke's lab
LacZ fusion
pGP830 (in pAC7), available in Jörg Stülke's lab
References
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Time of last update: 2025-04-05 10:24:09
Author of last update: Melvin.boenninger