yitJ

yitJ
168

bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase

Locus
BSU_11010
Molecular weight
67.75 kDa
Isoelectric point
5.34
Protein length
612 aaSequenceBlast
Gene length
1839 bpSequenceBlast
Function
methionine biosynthesis, tetrahydrofolate interconversion
Product
bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase
Essential
no
E.C.
2.1.1.13
Synonyms
yitJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0685 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,178,757  1,180,595
The protein
Catalyzed reaction/ biological activity
S-methyl-L-methionine + L-homocysteine --> 2 L-methionine (according to UniProt)
5-methyltetrahydrofolate + NAD(P)+ --> 5,10-methylenetetrahydrofolate + NAD(P)H (according to UniProt)
Protein family
C-terminal part: methylenetetrahydrofolate reductase family (single member, according to UniProt)
Domains
Hcy-binding domain (aa 1-280) (according to UniProt)
Cofactors
FAD (according to UniProt)
Zn2+ (according to UniProt)
Structure
1Q7M (PDB) (the methionine synthase domain, from Thermotoga maritima, 31% identity) PubMed
6FNU (PDB) (the methylene THF reductase domain, from Saccharomyces cerevisiae, 25% identity)
O06745 (AlphaFold)
Localization
membrane associated PubMed
Expression and Regulation
Operons
Genes
yitJ
Description
PubMed
Regulation
induced by methionine starvation (S-box) PubMed
the S-box RNA is degraded by RNase Y PubMed
Regulatory mechanism
S-box: RNA switch, PubMed, in S-box
Open in new tab

yitJ

2025-04-06 03:55:55

Bzhu

77

2289c9e44d66c9a8bc58d410f5e99cfd42a78a2f

C70131650850687D9161955DFBB754F29AD58BEA

Biological materials
Mutant
MGNA-B195 (yitJ::erm), available at the NBRP B. subtilis, Japan
BKE11010 (yitJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT,  downstream forward: _UP4_TAATTTCCAAAAGACTGCCT
BKK11010 (yitJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTCTGCCTCCTTTATT,  downstream forward: _UP4_TAATTTCCAAAAGACTGCCT
References
Other publications
Loading
The [[protein|S-box]]
Loading

41AB43191DDB2E0698D92FFD1ECEF45100F1F3F0

Page visits: 3774

Time of last update: 2025-04-06 03:56:07

Author of last update: Jstuelk