bkdR
168

transcriptional activator of the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon

Locus

BSU_24100

Molecular weight

77.75 kDa

Isoelectric point

6.24

Protein length

692 aaSequenceBlast

Gene length

2079 bpSequenceBlast

Function

regulation of branched-chain amino acid utilization

Product

transcriptional activator (for SigL-dependent promoter)

Essential

Synonyms

bkdR, yqiR

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3829 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

2,504,789 2,506,867

Phenotypes of a mutant

cold-sensitive PubMed

The protein

Protein family

Transcription factors activating transcription at SigL-dependent promoters

Domains

2 PAS domains (aa 115-185, aa 234-305) (according to UniProt)

Sigma-54 factor interaction domain (aa 368-598) (according to UniProt)

Structure

1OJL (PDB) (ZraR from Salmonella typhimurium, C-terminal interaction domain, corresponds to aa 368 ... 683 of BkdR, 41% identity) PubMed

P54529 (AlphaFold)

Expression and Regulation

Operons

Genes

bkdR

Description

PubMed

Regulation

repressed during growth in the presence of branched chain amino acids (CodY) PubMed

Regulatory mechanism

CodY: repression, PubMed, in codY regulon

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Biological materials

Mutant

MGNA-C375 (yqiR::erm), available at the NBRP B. subtilis, Japan

1A913 ( bkdR::kan), PubMed, available at BGSC and in Jörg Stülke's lab

BKE24100 (bkdR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCGATACCCCTTTGTA, downstream forward: _UP4_TAATTTGCAGAATAAACGCA

BKK24100 (bkdR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCGATACCCCTTTGTA, downstream forward: _UP4_TAATTTGCAGAATAAACGCA

References

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Time of last update: 2025-04-06 17:40:04

Author of last update: Melvin.boenninger

bkdR 168

bkdR
168