ytoI

ytoI
168

similar to transcriptional regulator (GntR family)

Locus
BSU_29270
Molecular weight
48.87 kDa
Isoelectric point
6.59
Protein length
439 aaSequenceBlast
Gene length
1320 bpSequenceBlast
Function
unknown
Product
unknown
Essential
no
Synonyms
ytoI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4109 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,997,301 → 2,998,620
Phenotypes of a mutant
an ytoI point mutation has been isolated upon selction at low pressure PubMed
The protein
Protein family
GntR family of transcription factors
Domains
2 CBS domains (aa 195-254, aa 256-314) (according to UniProt)
Structure
O34921 (AlphaFold)
Expression and Regulation
Operons
Genes
ytoI
Description
PubMed
Additional information
An ncRNA is predicted for'ytoI' PubMed
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ytoI

2025-03-16 17:24:51

ghost

91

123fdf57b313ace2ec7afdaaf309a23803e21280

37BEE6BD09E56AD5B14DED52E35B52F6D28EF21C

Biological materials
Mutant
MGNA-A152 (ytoI::erm), available at the NBRP B. subtilis, Japan
GP2213 (''ytoI::aphA3''), available in Jörg Stülke's lab
GP2214 (''ytoI::phleo''), available in Jörg Stülke's lab
BKE29270 (ΔytoI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG,  downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG
BKK29270 (ΔytoI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCCTTTCACCTCTAAGG,  downstream forward: _UP4_AGCTAAGGGACGTTCAGGCG
Expression vectors
pGP2933 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
References
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Page visits: 4150

Time of last update: 2025-04-06 05:56:17

Author of last update: Jstuelk