whiA

whiA
168

similar to transcription repressor of the yvcJ-glmR-whiA operon, involved in Z ring assembly, required for normal chromosome segregation

Locus
BSU_34750
Molecular weight
36.18 kDa
Isoelectric point
9.48
Protein length
316 aaSequenceBlast
Gene length
951 bpSequenceBlast
Function
control of Z ring asembly and chromosome segregation
Product
Cell division protein
Essential
no
Synonyms
whiA, yvcL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1481 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,569,573  3,570,523
Phenotypes of a mutant
a whiA zapA double mutant grows poorly, and the cells are filamentous (due to a defect in Z ring assembly) PubMed
a whiA ezrA double mutant grows poorly, and the cells are filamentous PubMed
a whiA minC-minD mutant grows poorly, and the cells are filamentous PubMed
a whiA noc double mutant grows poorly, and the cells are filamentous PubMed
a whiA parB double mutant is not viable, this can be suppressed by inactivation of yneA PubMed
a whiA yabA double mutant is not viable PubMed
the defects of the ''whiA zapA double mutant can be suppressed by inactivation of either gtaB, pgcA, or ugtP'' PubMed
highly sensitive for DNA damaging agents PubMed
defective in swarming motility (reduced rate of colony expansion) PubMed
The protein
Catalyzed reaction/ biological activity
in Enterococcus faecalis: transcription repressor of the yvcJ-glmR-whiA operon PubMed
Protein family
WhiA family (single member, according to UniProt) PubMed
Domains
suggested HTH domain at the N terminus PubMed
Structure
3HYI (PDB) (Crystal structure of full-length DUF199/WhiA from Thermotoga maritima)
3HYJ (PDB) (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)
O06975 (AlphaFold)
Localization
localizes to the nucleoid PubMed
Expression and Regulation
Operons
Genes
nahA-yvcJ-glmR-whiA-crh-yvcN
Description
PubMed
Regulation
constitutive expression at both protein and RNA levels PubMed
Expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) (WhiA) PubMed
Regulatory mechanism
WhiA: transcription repression, described for Enterococcus faecalis PubMed, in whiA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
A ncRNA is predicted between 'yvcI' and 'TrxB' PubMed
Open in new tab

nahA→yvcN

2025-04-04 14:43:19

Jstuelk

155

BCC5699F6256B8A1B7FD8A979DBBBC9D4D1198AB

9244FB113E998572106D983A19AB3AC81B9F9D35

Biological materials
Mutant
MGNA-B639 (yvcL::erm), available at the NBRP B. subtilis, Japan
GP231 (''amyE::PrpsJ-lacZ, whiA::cat'') available in Jörg Stülke's lab; plasmid pGP1468 was used to disrupt the gene.
BKE34750 (ΔwhiA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAGCCACCTCATTTCA,  downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
BKK34750 (ΔwhiA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAGCCACCTCATTTCA,  downstream forward: _UP4_TAACCGTAAAGAAAAGGGGA
Expression vectors
pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG), available in Jörg Stülke's lab
pGP2429 (N-terminal Strep-Tag, purification from E. coli and/or B. subtilis, in pDG148), for SPINE, available in Jörg Stülke's lab
pGP2430 (C-terminal Strep-Tag, purification from E. coli and/or B. subtilis, in pDG148), for SPINE, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's and Boris Görke's labs
FLAG-tag construct
GP782 (spc, based on pGP1331), available in Jörg Stülke's lab
Antibody
available in Leendert Hamoen's lab PubMed
References
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1986DB3078FBA16D6ACCD70EEAC95C46564D0958

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Time of last update: 2025-04-06 10:01:29

Author of last update: Jstuelk