nagR

nagR
168

transcriptional regulator (GntR family)

Locus
BSU_35030
Molecular weight
27.36 kDa
Isoelectric point
6.77
Protein length
243 aaSequenceBlast
Gene length
732 bpSequenceBlast
Function
regulator of the nagA-nagB-nagR operon
Product
transcriptional regulator (GntR family)
Essential
no
Synonyms
nagR, yvoA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2188 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,597,289  3,598,020
The protein
Catalyzed reaction/ biological activity
transcription repression of ''nagP and of the putative nagA-nagB-nagR'' operon in the absence of N-acetylglycosamine PubMed, weak repression of ''yflG'' PubMed
Protein family
GntR family of transcription factors
Domains
HTH gntR-type domain (aa 9-77) (according to UniProt)
Structure
2WV0 (PDB) PubMed
4U0V (PDB) (complex with glucosamine-6-phosphate) PubMed
4U0W (PDB) (complex with N-acetylglucosamine-6-phosphate) PubMed
4WWC (PDB) (complex with palindromic operator DNA) PubMed
O34817 (AlphaFold)
Effectors of protein activity
N-acetylglucosamine-6-phosphate and glucosamine-6-phosphate act as the molecular inducers PubMed
Kinetic information
K(D) for N-acetylglucosamine-6-phosphate: 1 mM PubMed
Paralogous protein(s)
TreR, YmfC, GamR, GmuR, FrlR, YydK
Expression and Regulation
Operons
Genes
nagA-nagB-nagR
Description
PubMed
Regulation
induced in the presence of N-acetylglucosamine (NagR) PubMed
Regulatory mechanism
NagR: repression, PubMed, in nagR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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nagA→nagR

2025-04-04 08:11:11

ghost

118

dfb9f60c6cb4712882f6e111396691335972d607

2CE10C0E65E696F5F9B1AAC17ED9D19E163A6ECF

Biological materials
Mutant
MGNA-A335 (yvoA::erm), available at the NBRP B. subtilis, Japan
BKE35030 (nagR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTCAGCATGTTCCTT,  downstream forward: _UP4_TCATAAAAAAAGCCTCCAAC
BKK35030 (nagR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTCAGCATGTTCCTT,  downstream forward: _UP4_TCATAAAAAAAGCCTCCAAC
References
Reviews
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Original Publications
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6D36D6360FE1CBFF5F64B2A6D1406405D6C6F7D5

Page visits: 5452

Time of last update: 2025-04-07 01:47:03

Author of last update: Melvin.boenninger