ilvH

ilvH
168

acetolactate synthase (small subunit)

Locus
BSU_28300
Molecular weight
19.31 kDa
Isoelectric point
10.3
Protein length
172 aaSequenceBlast
Gene length
519 bpSequenceBlast
Function
biosynthesis of branched-chain amino acids
Product
acetolactate synthase (small subunit)
Essential
no
E.C.
2.2.1.6
Synonyms
ilvH, ilvN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0440 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,894,733  2,895,251
The protein
Catalyzed reaction/ biological activity
H+ + 2 pyruvate --> (2S)-2-acetolactate + CO2 (according to UniProt)
Protein family
acetolactate synthase small subunit family (single member, according to UniProt)
Domains
ACT domain (aa 4-79) (according to UniProt)
Structure
2F1F (PDB) (regulatory subunit from Escherichia coli, 37% identity, 62% similarity) PubMed
P37252 (AlphaFold)
Effectors of protein activity
subject to feedback inhibition by valine PubMed
Expression and Regulation
Operons
Genes
ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD
Description
PubMed
Regulation
expression is stimulated in the presence of glucose (CcpA) PubMed
Regulatory mechanism
T-box: RNA switch, via tRNA controlled RNA switch, repression by BCAA, in T-box
TnrA: repression, PubMed, in tnrA regulon
CodY: repression, PubMed PubMed, in codY regulon
CcpA: activation, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
An antisense RNA is predicted for leuA PubMed
the T-box RNA is degraded by RNase Y PubMed
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ilvB→leuD

2025-03-01 21:15:25

Jstuelk

144

905a0fc4b9e1945f65cf0820ea8bda45e4ac716c

931C13BE07545B8037A118810CF4C8E29210A7CE

Biological materials
Mutant
BKE28300 (ilvH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTCAATGTGATAATTC,  downstream forward: _UP4_TAAAACATAACAAGGGAGAG
BKK28300 (ilvH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTCAATGTGATAATTC,  downstream forward: _UP4_TAAAACATAACAAGGGAGAG
References
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Page visits: 5526

Time of last update: 2025-03-14 05:17:49

Author of last update: Melvin.boenninger