murF
168

UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine synthetase

Locus

BSU_04570

Molecular weight

49.56 kDa

Isoelectric point

5.34

Protein length

457 aaSequenceBlast

Gene length

1374 bpSequenceBlast

Function

peptidoglycan precursor biosynthesis

Product

UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6- diaminopimeloyl-D-alanyl-D-alanine synthetase

Essential

yes

E.C.

6.3.2.10

Synonyms

murF, ydbQ

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0770 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

509,384 → 510,757

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

ATP + D-alanyl-D-alanine + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysine --> ADP + H⁺ + phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-L-lysyl-D-alanyl-D-alanine (according to UniProt)

Protein family

MurCDEF family (according to UniProt)

Structure

1GG4 (PDB) (from Escherichia coli, 32% identity, 50% similarity) PubMed

P96613 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)

forms banded patterns along the length of the cell, this pattern formation depends on MreB PubMed

Expression and Regulation

Operons

Genes

ddl-murF

Description

PubMed

Regulation

expression is reduced in a SigV mutant PubMed

the leader mRNA is processed upstream of ddl by RNase Y, this requires the RicA-RicF-RicT complex PubMed

Sigma factors

SigM: sigma factor, PubMed, in sigM regulon

SigV: sigma factor, in sigV regulon

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Biological materials

Mutant

MGNA-C105 (murF::erm), available at the NBRP B. subtilis, Japan

References

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Time of last update: 2025-03-14 15:16:46

Author of last update: Melvin.boenninger

murF 168

murF
168