azoR1

azoR1
168

azoreductase, involved in quinone detoxification

Locus
BSU_19230
Molecular weight
22.83 kDa
Isoelectric point
4.99
Protein length
208 aaSequenceBlast
Gene length
627 bpSequenceBlast
Function
quinone detoxification
Product
azoreductase
Essential
no
Synonyms
azoR1, yocJ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1182 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,096,350 2,096,976
The protein
Catalyzed reaction/ biological activity
anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD+ --> 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H+ + 2 NADH (according to UniProt)
Protein family
azoreductase type 1 family (with AzoR2, according to UniProt)
Structure
3P0R (PDB) (from B. anthracis, 64% identity, 81% similarity)
O35022 (AlphaFold)
Paralogous protein(s)
AzoR2
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
azoR1
Description
PubMed
Regulation
induced by thiol-reactive compounds (catechol, menaquinone) (YodB) PubMed
Regulatory mechanism
YodB: repression, PubMed, in yodB regulon
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azoR1

2025-02-26 05:01:29

Bzhu

92

3010a253791488a0c14278049a20c80502f26844

8881E3C43D39AF052197A1E21C2D9298F5FD8D97

Biological materials
Mutant
MGNA-B415 (yocJ::erm), available at the NBRP B. subtilis, Japan
BKE19230 (azoR1::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGTATTCCTCCCGGTA, downstream forward: _UP4_TAATAAAGCCAAGACTCCTA
BKK19230 (azoR1::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTGTATTCCTCCCGGTA, downstream forward: _UP4_TAATAAAGCCAAGACTCCTA
References
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Page visits: 3107

Time of last update: 2025-03-15 08:31:29

Author of last update: Melvin.boenninger