nrgA

nrgA
168

ammonium transporter, required at low ammonium concentration

Locus
BSU_36510
Molecular weight
42.57 kDa
Isoelectric point
5.73
Protein length
404 aaSequenceBlast
Gene length
1215 bpSequenceBlast
Function
ammonium uptake
Product
ammonium transporter
Essential
no
Synonyms
nrgA, amtB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0004 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, TnrA regulon

Gene
Coordinates
3,756,790  3,758,004
The protein
Protein family
ammonia transporter channel (TC 1.A.11.2) family (single member, according to UniProt)
Structure
4NH2 (PDB) (from E. coli) PubMed
Q07429 (AlphaFold)
Localization
cell membrane (according to UniProt),  membrane
Expression and Regulation
Operons
Genes
nrgA-nrgB
Description
PubMed
Regulation
induced by ammonium limitation
Regulatory mechanism
TnrA: activation, PubMed, in tnrA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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nrgA→nrgB

2025-03-16 03:17:22

ghost

99

f7f99c18cba030e60508f26bef1662bcb7a80522

89C9A9EC5C318E8075D00EF7FA51F420FFDBD2A5

Biological materials
Mutant
GP4167 (ΔnrgA-nrgB::spec), available in  Jörg Stülke's lab
GP739 (cat), GP255 (ΔnrgA-nrgB, cat), both available in Jörg Stülke's lab PubMed
1A920 ( nrgA::erm), PubMed, available at BGSC
BKE36510 (nrgA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT,  downstream forward: _UP4_GATTCTATGTGAGGAGTGAC
BKK36510 (nrgA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCTCATTCCTCCGTAT,  downstream forward: _UP4_GATTCTATGTGAGGAGTGAC
LacZ fusion
pGP168 (in pAC7), available in Jörg Stülke's lab PubMed
Labs working on this gene/protein
Susan Fisher, Boston, USA homepage
References
Reviews
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Original Publications
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796BD46066B1E6147048664FA78679B42FA9D628

Page visits: 6137

Time of last update: 2025-03-17 03:48:03

Author of last update: Jstuelk