tcyP

tcyP
168

cystine and diaminopimelate transporter

Locus
BSU_09130
Molecular weight
48.82 kDa
Isoelectric point
9.47
Protein length
463 aaSequenceBlast
Gene length
1392 bpSequenceBlast
Function
cystine and diaminopimelate uptake
Product
cystine and diaminopimelate transporter
Essential
no
Synonyms
tcyP, yhcL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1823 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
986,986 → 988,377
The protein
Catalyzed reaction/ biological activity
cystine-proton symporter PubMed
transport of diaminopimelate PubMed
Protein family
dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family (according to UniProt)
Structure
3KBC (PDB) (from Pyrococcus horikoshii, 28% identity) PubMed
P54596 (AlphaFold)
Kinetic information
K(m) = 0.6 myM cystine PubMed
Localization
membrane associated PubMed
Expression and Regulation
Operons
Genes
tcyP
Regulation
repressed in the presence of sulfate or cysteine (CymR) PubMed
Regulatory mechanism
CymR: repression, PubMed, in cymR regulon
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tcyP

2025-03-11 05:59:32

ghost

103

5ea1d8626a0599a33a06a2dd344d1eb12bf3becd

1498CA448F071BF799C224B19965903939D1377D

Biological materials
Mutant
GP4481 (trpC2 ΔtcyP::neo), available in Jörg Stülke's lab
GP4678 (trpC2 ΔtcyP::lox72), available in Jörg Stülke's lab
GP4680 (trpC2 ΔtcyP::ermC), available in Jörg Stülke's lab
MGNA-A657 (yhcL::erm), available at the NBRP B. subtilis, Japan
1A949 ( tcyP::spec), PubMed, available at BGSC
BKE09130 (ΔtcyP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC,  downstream forward: _UP4_TAACATATGAAAACGTGTAA
BKK09130 (ΔtcyP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGCAGGTAAACTCTCCC,  downstream forward: _UP4_TAACATATGAAAACGTGTAA
Labs working on this gene/protein
[[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France
References
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EC287BBD7AB1EB44A33BF29144448F53D06AC6D9

Page visits: 5493

Time of last update: 2025-03-13 05:08:49

Author of last update: Robert.warneke