lcfA

lcfA
168

long chain acyl-CoA synthetase, involved in surfactin production

Locus
BSU_28560
Molecular weight
62.52 kDa
Isoelectric point
6.12
Protein length
560 aaSequenceBlast
Gene length
1683 bpSequenceBlast
Function
fatty acid degradation
Product
long chain acyl-CoA synthetase
Essential
no
E.C.
6.2.1.3
Synonyms
lcfA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, FadR regulon

Gene
Coordinates
2,918,646  2,920,328
The protein
Catalyzed reaction/ biological activity
long-chain fatty acid + ATP + CoA --> long-chain fatty acyl-CoA + AMP + diphosphate (according to UniProt)
activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
Protein family
ATP-dependent AMP-binding enzyme family (according to UniProt)
Structure
3R44 (PDB) (Very-long-chain fatty acyl-CoA synthetase from Mycobacterium tuberculosis, 28% identity) PubMed
P94547 (AlphaFold)
Paralogous protein(s)
LcfB, YhfT, YngI
Localization
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
lcfA-fadR-fadB-etfB-etfA
Description
PubMed
Regulation
induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed
Regulatory mechanism
FadR: repression, PubMed, in fadR regulon
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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lcfA→etfA

2025-03-11 06:55:54

Jstuelk

101

d18d49394aac4cec0002a2695f8ea4b1c4cda4f5

DEE7D2778BB88C7028C79F5FEDED5CD757497033

Biological materials
Mutant
available in Mohamed Marahiel's lab PubMed
1A852 ( lcfA::cat), PubMed, available at BGSC
BKE28560 (lcfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA,  downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
BKK28560 (lcfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAACCTCCCCTTTCGA,  downstream forward: _UP4_TAAAAAAAGAGAACGCCTAT
References
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7AC5217CA35F8E134ABB35D74F1BA5E684D2E058

Page visits: 6419

Time of last update: 2025-03-15 19:09:34

Author of last update: Melvin.boenninger