mtnD

mtnD
168

1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase

Locus
BSU_13620
Molecular weight
20.68 kDa
Isoelectric point
4.41
Protein length
178 aaSequenceBlast
Gene length
537 bpSequenceBlast
Function
methionine salvage
Product
1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Essential
no
Synonyms
mtnD, ykrZ
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1791 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,429,584 1,430,120
The protein
Catalyzed reaction/ biological activity
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 --> 3-(methylsulfanyl)propanoate + CO + formate + 2 H+ (according to UniProt)
1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 --> 4-methylsulfanyl-2-oxobutanoate + formate + 2 H+ (according to UniProt)
Protein family
acireductone dioxygenase (ARD) family (single member, according to UniProt)
Structure
4QGL (PDB) (from B. anthracis, 71% identity)
O31669 (AlphaFold)
Additional information
belongs to the 100 most abundant proteins PubMed
Expression and Regulation
Operons
Genes
mtnW-mtnX-mtnB-mtnD
Description
PubMed
Regulation
induced by methionine starvation (S-box) PubMed
Regulatory mechanism
S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mtnW→mtnD

2025-03-02 23:55:30

Jstuelk

80

a2b13ba83890892f9d8595d5e452be227045e93c

6416B0A5CDDC144986D3159AFC5B04D4507F2AE1

Biological materials
Mutant
MGNA-A786 (ykrZ::erm), available at the NBRP B. subtilis, Japan
BKE13620 (mtnD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATTCCTCCTTTTA, downstream forward: _UP4_TAAGCGTGAGATAGCCCCGT
BKK13620 (mtnD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTCAATTCCTCCTTTTA, downstream forward: _UP4_TAAGCGTGAGATAGCCCCGT
References
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C7D9E701B2CA2C9E85D69CAD6E0A45BE590BAED9

Page visits: 5447

Time of last update: 2025-03-14 11:16:57

Author of last update: Melvin.boenninger