pckA

pckA
168

phosphoenolpyruvate carboxykinase

Locus
BSU_30560
Molecular weight
58.13 kDa
Isoelectric point
5.12
Protein length
527 aaSequenceBlast
Gene length
1584 bpSequenceBlast
Function
synthesis of phosphoenolpyruvate
Product
phosphoenolpyruvate carboxykinase
Essential
no
E.C.
4.1.1.49
Synonyms
pckA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1866 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,129,530  3,131,113
The protein
Catalyzed reaction/ biological activity
ATP + oxaloacetate --> ADP + CO2 + phosphoenolpyruvate (according to UniProt)
Protein family
phosphoenolpyruvate carboxykinase (ATP) family (single member, according to UniProt)
Domains
Nucleotide binding Domain (233240)
Structure
1J3B (PDB) (from Thermus thermophilus, 55.1% identity) PubMed
P54418 (AlphaFold)
Localization
cytoplasm (according to Swiss-Prot),  cytoplasm
Additional information
PckA is degraded in the forespore using MdfA as the adaptor protein PubMed
Expression and Regulation
Operons
Genes
pckA
Description
PubMed
Regulation
repressed by glucose (35-fold) CcpN PubMed
Regulatory mechanism
CcpN: repression, PubMed, in ccpN regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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pckA

2025-03-02 00:42:15

ghost

92

c51496c43c3d7c242b6ec156cd659276ffd6d4b4

709069FD604114640A936EAD2D10F54B494E230C

Biological materials
Mutant
1A1005 ( pckA::spec), PubMed, available at BGSC
1A996 ( pckA::spec), PubMed, available at BGSC
GP1147 (pckA::neo), available in Jörg Stülke's lab
BKE30560 (pckA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAAACCTTCCTTTAT,  downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG
BKK30560 (pckA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGAAACCTTCCTTTAT,  downstream forward: _UP4_TAAAAAACAAAAGCCAAGAG
Expression vectors
pGP1753 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab) PubMed
pGP1762 (for expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab)
pGP1763 (for expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab)
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1129 (spc, based on pGP1331), available in Jörg Stülke's lab PubMed
LacZ fusion
pGP3312 (cat, based on pAC5), available in Jörg Stülke's lab
GFP fusion
GP1430 (spc, based on pGP1870), available in Jörg Stülke's lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
References
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Time of last update: 2025-03-11 01:20:05

Author of last update: Jstuelk