skfE

skfE
168

ABC transporter (ATP-binding protein), export of the spore killing factor

Locus
BSU_01950
Molecular weight
26.75 kDa
Isoelectric point
6.34
Protein length
239 aaSequenceBlast
Gene length
720 bpSequenceBlast
Function
export of the spore killing factor SkfA
Product
ABC transporter (ATP-binding protein)
Essential
no
E.C.
3.6.3.25
Synonyms
skfE, ybdA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1131 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
216,913  217,632
Phenotypes of a mutant
inactivation of ''skfE'' reduces sporulation efficiency to 54% that of wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
ATP + H2O + sulfate --> ADP + H+ + phosphate + sulfate (according to UniProt)
ATP + H2O + thiosulfate --> ADP + H+ + phosphate + thiosulfate (according to UniProt)
Protein family
ABC transporter superfamily (according to UniProt)
Domains
ABC transporter domain (aa 4-232) (according to UniProt)
Structure
4RVC (PDB) (from Geobacillus kaustophilus, 33% identity) PubMed
O31427 (AlphaFold)
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
skfA-skfB-skfC-skfE-skfF-skfG-skfH
Description
PubMed
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
AbrB: repression, PubMed, in abrB regulon
PhoP: activation, PubMed, in phoP regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
Northern blotting during during phosphate limitation showed an intense 0.25 kb 'skfA'-specific transcript, and a weaker 6.5 kb 'skfA-skfB-skfC-skfE-skfF-skfG-skfH' transcript.
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skfA→skfH

2025-03-16 16:39:46

ghost

162

f96cb932f6aa46632dc613334e3526e3904b2534

E1A5B1DE7F5A4653FA0FC2E7FE7AAD3454DBFF28

Biological materials
Mutant
MGNA-C515 (ybdA::erm), available at the NBRP B. subtilis, Japan
BKE01950 (skfE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA,  downstream forward: _UP4_TAATATCATTTCTTTTTTAT
BKK01950 (skfE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGCATCAAATCTTCTCCTA,  downstream forward: _UP4_TAATATCATTTCTTTTTTAT
References
Reviews
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Original Publications
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5886364199844356446C08F08664B301A714E74A

Page visits: 4196

Time of last update: 2025-03-21 09:05:54

Author of last update: Jstuelk