putB
168
proline dehydrogenase (L-proline, FAD)
Locus
BSU_03200
Molecular weight
34.89 kDa
Isoelectric point
7.29
Function
proline utilization
Product
proline dehydrogenase (L-proline, FAD)
Essential
no
E.C.
1.5.99.8
Synonyms
putB, ycgM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0506 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
344,551 345,462
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
quinone + L-proline --> (S)-1-pyrroline-5-carboxylate + quinol + H+ (according to UniProt)
Protein family
proline dehydrogenase family (with FadM, according to UniProt)
FAD (according to UniProt)
Structure
4H6Q (PDB) (from Deinococcus radiodurans , 42% identity) PubMed
Paralogous protein(s)
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B992 (ycgM::erm), available at the NBRP B. subtilis, Japan
Expression vectors
pGP3732: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
References
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Time of last update: 2025-03-18 07:36:11
Author of last update: Robert.warneke