putB

putB
168

proline dehydrogenase (L-proline, FAD)

Locus
BSU_03200
Molecular weight
34.89 kDa
Isoelectric point
7.29
Protein length
303 aaSequenceBlast
Gene length
912 bpSequenceBlast
Function
proline utilization
Product
proline dehydrogenase (L-proline, FAD)
Essential
no
E.C.
1.5.99.8
Synonyms
putB, ycgM
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0506 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
344,551  345,462
Phenotypes of a mutant
no growth with proline as single source of carbon or nitrogen PubMed
inactivation of ''putB'' eliminates sporulation PubMed
The protein
Catalyzed reaction/ biological activity
quinone + L-proline --> (S)-1-pyrroline-5-carboxylate + quinol + H+ (according to UniProt)
Protein family
proline dehydrogenase family (with FadM, according to UniProt)
Cofactors
FAD (according to UniProt)
Structure
4H6Q (PDB) (from Deinococcus radiodurans , 42% identity) PubMed
P94390 (AlphaFold)
Paralogous protein(s)
FadM
Expression and Regulation
Operons
Genes
putB-putC-putP
Description
PubMed
Regulation
expressed under conditions that trigger sporulation (Spo0A) PubMed
Regulatory mechanism
PutR: activation, PubMed, in putR regulon
Spo0A: activation, PubMed, in spo0A regulon
CodY: repression, displacement of PutR PubMed, in codY regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
overexpressed in a pnpA mutant PubMed
the putP part of the mRNA is cleaved by RNase III PubMed
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putB→putP

2025-03-16 14:52:55

Jstuelk

116

d991744c53f7fc7228712ba483de8c17cc05c88a

72CAEF916BFA077D3E9623FC66EECF026978B0D8

Biological materials
Mutant
GP3904 (''putB-putC::aphA3''), available in Jörg Stülke's lab
MGNA-B992 (ycgM::erm), available at the NBRP B. subtilis, Japan
BKE03200 (putB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTCCAACCTCAACACCC,  downstream forward: _UP4_AAGAAGTAAAAAAGGAGAGA
BKK03200 (putB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTCCAACCTCAACACCC,  downstream forward: _UP4_AAGAAGTAAAAAAGGAGAGA
Expression vectors
pGP3732: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
References
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Page visits: 6551

Time of last update: 2025-03-17 16:45:58

Author of last update: Robert.warneke