ycgT

ycgT
168

NADPH:ferredoxin oxidoreductase

Locus
BSU_03270
Molecular weight
36.81 kDa
Isoelectric point
5.61
Protein length
336 aaSequenceBlast
Gene length
1011 bpSequenceBlast
Function
unknown
Product
NADPH:ferredoxin oxidoreductase
Essential
no
Synonyms
ycgT
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0492 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
352,858 → 353,868
The protein
Catalyzed reaction/ biological activity
H+ + NADP+ + 2 reduced [2Fe-2S]-[ferredoxin] --> NADPH + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
Protein family
ferredoxin--NADP reductase type 2 family (with YumC, according to UniProt)
Cofactors
FAD PubMed
Structure
5XHU (PDB)
O31475 (AlphaFold)
Paralogous protein(s)
YumC
Additional information
The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin
reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. PubMed
Expression and Regulation
Operons
Genes
ycgT
Description
PubMed
Regulation
induced under conditions of iron starvation (Fur) PubMed
Regulatory mechanism
Fur: repression, PubMed, in fur regulon
Additional information
the mRNA is very stable (half-life > 15 min) PubMed
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ycgT

2025-02-24 23:27:16

Jstuelk

77

051e8ac5689d8158f132195c7420e821fb6b8f43

A7049AD5890F7B0CC6E9E3A7AB7C2DB2BDBF17C4

Biological materials
Mutant
MGNA-C052 (ycgT::erm), available at the NBRP B. subtilis, Japan
GP1852 (ΔycgT::cat), available in Jörg Stülke's lab
BKE03270 (ΔycgT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT,  downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
BKK03270 (ΔycgT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT,  downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
References
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Page visits: 4069

Time of last update: 2025-03-11 14:36:09

Author of last update: Melvin.boenninger