carA

carA
168

carbamoyl-P transferase (arginine) (subunit A)

Locus
BSU_11230
Molecular weight
38.90 kDa
Isoelectric point
6.21
Protein length
353 aaSequenceBlast
Gene length
1062 bpSequenceBlast
Function
biosynthesis of arginine
Product
carbamoyl-P transferase (arginine) (subunit A)
Essential
no
E.C.
6.3.5.5
Synonyms
carA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0505 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,199,327  1,200,388
The protein
Catalyzed reaction/ biological activity
2 ATP + H2O + bicarbonate + L-glutamine --> 2 ADP + carbamoyl-P + 2 H+ + L-glutamate + phosphate (according to UniProt)
Protein family
CarA family (with PyrAA, according to UniProt)
Domains
Glutamine amidotransferase type-1 domain (aa 163-349) (according to UniProt)
Structure
1JDB (PDB) (from Escherichia coli, 47% identity, 64% similarity) PubMed
P36838 (AlphaFold)
Paralogous protein(s)
PyrAA
Expression and Regulation
Operons
Genes
argC-argJ-argB-argD-carA-carB-argF
Description
PubMed
Regulation
repressed by arginine (AhrC) PubMed
induced in the absence of RnpM PubMed
Regulatory mechanism
AhrC: repression, PubMed, in ahrC regulon
CodY: repression, PubMed, in codY regulon
RnpM: unknown, PubMed, in rnpM regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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argC→argF

2025-03-05 00:58:55

Jstuelk

120

bd8576e7d6b5ef98ec61ff0587a9708cd8d39155

DA268D208C03E6C5DAF0361E4714668AC025F3EA

Biological materials
Mutant
BKE11230 (carA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAACAGCCCACCTCTT,  downstream forward: _UP4_ATACCAGCAAGGAGAGAAAT
BKK11230 (carA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTAACAGCCCACCTCTT,  downstream forward: _UP4_ATACCAGCAAGGAGAGAAAT
GP3714 (carA::aphA3 trpC2) available in the Stülke lab
Expression vectors
pGP3713: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
pGP3769: expression of Strep-carA by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
References
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4202EAE5D0B2A718382092423DD99E35FDE81218

Page visits: 5535

Time of last update: 2025-03-15 01:17:49

Author of last update: Jstuelk