yisK

yisK
168

oxaloacetate enol-keto tautomerase, modulator of Mbl activity, minor oxaloacetate decarboxylase activity

Locus
BSU_10750
Molecular weight
32.99 kDa
Isoelectric point
5.45
Protein length
301 aaSequenceBlast
Gene length
906 bpSequenceBlast
Function
detoxification of enol-oxaloacetate, control of cell division,
Product
oxaloacetate enol-keto tautomerase, modulator of Mbl activity
Essential
no
E.C.
5.3.2.2
Synonyms
yisK
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0179 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,152,244  1,153,149
Phenotypes of a mutant
overexpression results in loss of cell width and cell lysis (suppressed by mutations in Mbl that affect the ATP-binding site) PubMed
The protein
Catalyzed reaction/ biological activity
enol-oxaloacetate --> oxaloacetate PubMed
oxaloacetate --> pyruvate + CO2 PubMed
Protein family
FAH family (single member, according to UniProt)
Cofactors
Mn2+ PubMed
Structure
3R60 (PDB) (from Mycobacterium abscessus, 42% identity)
8SKY (PDB) PubMed
O06724 (AlphaFold)
Kinetic information
for oxaloacetate tautomerase activity
Km = 44 +/- 8 µM PubMed
vmax = 402 +/- 13 µmol min-1 mg-1 PubMed
for oxaloacetate decarboxylase activity
Km = 134 µM PubMed
Kcat = 31 min−1 PubMed
Localization
punctate and/or punctate/helical pattern, depends on Mbl PubMed
Expression and Regulation
Operons
Genes
yisK-yisL
Description
PubMed
Regulatory mechanism
Spo0A: activation, PubMed, in spo0A regulon
Sigma factors
SigH: sigma factor, PubMed, in sigH regulon
Open in new tab

yisK->yisL

2025-03-08 11:03:59

Bzhu

115

80c3941a8e5ceb3d3e90ebb4127c6814f250a978

AA9FDC136CBA646E187DAAE09D52B59CF2A9E17A

Biological materials
Mutant
MGNA-B291 (yisK::erm), available at the NBRP B. subtilis, Japan
BKE10750 (yisK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATTCCTCCATCATC,  downstream forward: _UP4_TGAATAAAACTGGAGGGCGG
BKK10750 (yisK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTATTCCTCCATCATC,  downstream forward: _UP4_TGAATAAAACTGGAGGGCGG
References
Loading

07E876715DCF551B8BDB6AA5D33C700696B4E730

Page visits: 6646

Time of last update: 2025-03-14 19:07:13

Author of last update: Jstuelk