araD

araD
168

L-ribulose-phosphate 4-epimerase

Locus
BSU_28780
Molecular weight
25.54 kDa
Isoelectric point
5.24
Protein length
229 aaSequenceBlast
Gene length
690 bpSequenceBlast
Function
arabinose utilization
Product
L-ribulose-phosphate 4-epimerase
Essential
no
E.C.
5.1.3.4
Synonyms
araD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG0235 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, AraR regulon

Gene
Coordinates
2,944,983  2,945,672
The protein
Catalyzed reaction/ biological activity
L-ribulose 5-phosphate --> D-xylulose 5-phosphate (according to UniProt)
Protein family
aldolase class II family (with MtnB, according to UniProt)
Structure
1K0W (PDB) (from   \t   Escherichia coli, 59% identity, 74% similarity)\tPubMed
P94525 (AlphaFold)
Expression and Regulation
Operons
Genes
araA-araB-araD-araL-araM-araN-araP-araQ-abfA
Description
PubMed
Regulation
induced by arabinose (AraR) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
AraR: repression, PubMed, in araR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
Additional information
the mRNA is very stable (half-life > 15 min) PubMed
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araA→abfA

2025-03-17 19:30:39

ghost

142

b7d3312ef868c449eb18db3a2b2bf36130567026

7BE2C086CD88758AEADFA2166B6FC6182EB55613

Biological materials
Mutant
BKE28780 (araD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAAGCATGCTCCATCACC,  downstream forward: _UP4_CATTTTTATCGAAAACACGG
BKK28780 (araD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCAAGCATGCTCCATCACC,  downstream forward: _UP4_CATTTTTATCGAAAACACGG
References
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3859854178E573AE8ACCE6DF85E32C495D316DA1

Page visits: 4339

Time of last update: 2025-03-17 17:36:46

Author of last update: Melvin.boenninger