mtlR

mtlR
168

transcriptional activator of the mtlA-mtlF-mtlD operon

Locus
BSU_04160
Molecular weight
78.67 kDa
Isoelectric point
5.31
Protein length
694 aaSequenceBlast
Gene length
2085 bpSequenceBlast
Function
regulation of mannitol utilization
Product
transcriptional activator, PRD-type
Essential
no
Synonyms
mtlR, ydaA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG3711 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
467,130  469,214
The protein
Catalyzed reaction/ biological activity
binding to the promoter region of the mtlA-mtlF-mtlD operon and activation of expression
Protein family
PRD-containing transcription factors
Domains
N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 aa 195-300 and PRD2 aa 305-410), EIIB (Gat)-like domain, EIIA (Mtl)-like domain
PTS EIIB domain type-2 (aa 413-502) (according to UniProt)
PTS EIIA domain type-2 (aa 536-683) (according to UniProt)
Structure
P96574 (AlphaFold)
Modification
phosphorylated on His-342 and/or His-399 by PtsH PubMed
phosphorylation on His-599 by MtlA PubMed
phosphorylation on Cys-419 by MtlF PubMed
Effectors of protein activity
activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed
Localization
upon interaction with non-phosphorylated MtlA, the active MtlR localizes to the membrane PubMed
Expression and Regulation
Operons
Genes
mtlR
Description
PubMed
Regulation
repressed by glucose (CcpA) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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mtlR

2025-03-11 06:34:26

ghost

74

94cbef71388f5fb1fe4cf9a5408dcb95147eee80

7B36DA36C65291ECB21349F02D2658BA28F7311D

Biological materials
Mutant
BKE04160 (mtlR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC,  downstream forward: _UP4_TAAACCTGCATGGCACACGT
BKK04160 (mtlR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAAAAGACCTCCTAGC,  downstream forward: _UP4_TAAACCTGCATGGCACACGT
References
Reviews
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Original Publications
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1CEDC98C9EDD8F3DEA0AF4B2104DA1BF86E688C2

Page visits: 4038

Time of last update: 2025-03-17 21:46:44

Author of last update: Jstuelk