ispC

ispC
168

1-deoxy-D-xylulose-5-phosphate reductoisomerase, second step in the MEP pathway of isoprenoid biosynthesis

Locus
BSU_16550
Molecular weight
43.12 kDa
Isoelectric point
5.8
Protein length
383 aaSequenceBlast
Gene length
1152 bpSequenceBlast
Function
MEP pathway of isoprenoid biosynthesis
Product
1-deoxy-D-xylulose-5-phosphate reductoisomerase
Essential
yes
E.C.
1.1.1.267
Synonyms
ispC, yluB, dxr
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0743 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,722,871  1,724,022
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
2-C-methyl-D-erythritol 4-phosphate + NADP+ --> 1-deoxy-D-xylulose 5-phosphate + H+ + NADPH (according to UniProt)
Protein family
DXR family (single member, according to UniProt)
Structure
1R0K (PDB) (from Zymomonas mobilis, 45% identity) PubMed
O31753 (AlphaFold)
Expression and Regulation
Operons
Genes
ispC-rasP-proS-polC
Description
PubMed
Open in new tab

ispC→polC

2025-03-15 05:39:42

Jstuelk

147

babdb9046d2f2d5bae4dac8092e2f7235f01c3e9

23B0B9EE13D6BD9F4D99911E7AA88933F4080154

Genes
uppS-cdsA-ispC-rasP-proS-polC
Description
PubMed
Open in new tab

uppS→polC

2025-03-09 14:34:03

Jstuelk

141

c6a20b58d42a343484ae9ff8f9312018bf0f82f2

3AF78B34597AE93B1AF63769A0A2561D91069003

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Expression vectors
pGP4000: expression of ispC by pBQ200 in B. subtilis, available in Jörg Stülke's lab
References
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Page visits: 5909

Time of last update: 2025-03-16 12:31:03

Author of last update: Robert.warneke