ispC

ispC
168

1-deoxy-D-xylulose-5-phosphate reductoisomerase, second step in the MEP pathway of isoprenoid biosynthesis

Locus
BSU_16550
Molecular weight
43.12 kDa
Isoelectric point
5.8
Protein length
383 aaSequenceBlast
Gene length
1152 bpSequenceBlast
Function
MEP pathway of isoprenoid biosynthesis
Product
1-deoxy-D-xylulose-5-phosphate reductoisomerase
Essential
yes
E.C.
1.1.1.267
Synonyms
ispC, yluB, dxr
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0743 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,722,871  1,724,022
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
2-C-methyl-D-erythritol 4-phosphate + NADP+ --> 1-deoxy-D-xylulose 5-phosphate + H+ + NADPH (according to UniProt)
Protein family
DXR family (single member, according to UniProt)
Structure
1R0K (PDB) (from Zymomonas mobilis, 45% identity) PubMed
O31753 (AlphaFold)
Expression and Regulation
Operons
Genes
ispC-rasP-proS-polC
Description
PubMed
Open in new tab

ispC→polC

2025-03-15 05:39:42

Jstuelk

147

babdb9046d2f2d5bae4dac8092e2f7235f01c3e9

23B0B9EE13D6BD9F4D99911E7AA88933F4080154

Genes
uppS-cdsA-ispC-rasP-proS-polC
Description
PubMed
Open in new tab

uppS→polC

2025-03-09 14:34:03

Jstuelk

141

c6a20b58d42a343484ae9ff8f9312018bf0f82f2

3AF78B34597AE93B1AF63769A0A2561D91069003

Additional information
the mRNA is cleaved by RNase III PubMed
Biological materials
Expression vectors
pGP4000: expression of ispC by pBQ200 in B. subtilis, available in Jörg Stülke's lab
References
Ding X, Zheng Z, Zhao G, Wang L, Wang H, Yang Q, Zhang M, Li L, Wang PBottom-up synthetic biology approach for improving the efficiency of menaquinone-7 synthesis in Bacillus subtilis.Microbial cell factories. 2022 May 28; 21(1):101. PMID: 35643569
DiChiara JM, Liu B, Figaro S, Condon C, Bechhofer DH Mapping of internal monophosphate 5' ends of Bacillus subtilis messenger RNAs and ribosomal RNAs in wild-type and ribonuclease-mutant strains. Nucleic acids research. 2016 Apr 20; 44(7):3373-89. doi:10.1093/nar/gkw073. PMID:26883633
Hess BM, Xue J, Markillie LM, Taylor RC, Wiley HS, Ahring BK, Linggi B Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics. PloS one. 2013; 8(6):e66104. doi:10.1371/journal.pone.0066104. PMID:23840410
Xue J, Ahring BK Enhancing isoprene production by genetic modification of the 1-deoxy-d-xylulose-5-phosphate pathway in Bacillus subtilis. Applied and environmental microbiology. 2011 Apr; 77(7):2399-405. doi:10.1128/AEM.02341-10. PMID:21296950
Julsing MK, Rijpkema M, Woerdenbag HJ, Quax WJ, Kayser O Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis. Applied microbiology and biotechnology. 2007 Jul; 75(6):1377-84. . PMID:17458547
Ricagno S, Grolle S, Bringer-Meyer S, Sahm H, Lindqvist Y, Schneider G Crystal structure of 1-deoxy-d-xylulose-5-phosphate reductoisomerase from Zymomonas mobilis at 1.9-A resolution. Biochimica et biophysica acta. 2004 Apr 08; 1698(1):37-44. . PMID:15063313

58CDF08A8F05C0BC25EB6E400316F85FD0F307E5

Page visits: 5910

Time of last update: 2025-03-16 12:48:30

Author of last update: Robert.warneke