ndhF

ndhF
168

subunit of a Na+:bicarbonate transporter MpsA-MpsB

Locus
BSU_01830
Molecular weight
55.30 kDa
Isoelectric point
10.15
Protein length
505 aaSequenceBlast
Gene length
1518 bpSequenceBlast
Function
uptake of bicarbonate
Product
subunit of a Na+:bicarbonate transporter
Essential
no
Synonyms
ndhF, ybxE, mpsA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1009 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
205,409 → 206,926
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
severe growth defect PubMed
inactivation of ndhF facilitates the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate PubMed
The protein
Catalyzed reaction/ biological activity
uptake of bicarbonate (with MpsB) PubMed
Protein family
complex I subunit 5 family (single member, according to UniProt)
Cofactors
FMN or FAD PubMed
Structure
4HE8 (PDB) (from Thermus thermophilus, corresponds to aa 70 ... 352, 28.% identity) PubMed
P39755 (AlphaFold)
Localization
cell membrane (according to UniProt)
Expression and Regulation
Operons
Genes
ndhF-ybcC-ybcF-ybcH-ybcI
Description
PubMed
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ndhF→ybcI

2025-03-10 03:43:47

Jstuelk

109

e140e954b90890d3138431566447fb6e0403ad92

FF8650333154CD6783C7E2C33588F0F5E9F40953

Additional information
highly expressed at high iron concentrations PubMed
Biological materials
Mutant
BKE01830 (ΔndhF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT,  downstream forward: _UP4_ATTTCATAAGGAGCTAATCT
BKK01830 (ΔndhF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTAAATTCTCCCTTTT,  downstream forward: _UP4_ATTTCATAAGGAGCTAATCT
References
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Time of last update: 2025-03-13 05:53:23

Author of last update: Jstuelk