pghB

pghB
168

phage-derived gamma polyglutamate hydrolase

Locus
BSU_12480
Molecular weight
22.70 kDa
Isoelectric point
6.42
Protein length
200 aaSequenceBlast
Gene length
603 bpSequenceBlast
Function
polyglutamate degradation
Product
phage-derived gamma polyglutamic acid hydrolase
Essential
no
Synonyms
pghB, yjqB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4195 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,319,011 → 1,319,613
The protein
Catalyzed reaction/ biological activity
hydrolysis of polyglutamic acid PubMed
Protein family
UPF0714 family (according to UniProt)
Domains
DUF867 x, [[gamma-PGA hydrolase domain]] PubMed
Structure
3A9L (PDB) (from B. subtilis phage NIT1, 41% identity) PubMed
O34785 (AlphaFold)
Paralogous protein(s)
PghC, PghL, PghZ
Expression and Regulation
Operons
Genes
pghB-yjqC
Description
PubMed
Open in new tab

pghB→yjqC

2025-02-26 14:12:27

Jstuelk

68

2c7da9c24cc326eb2f7f799b623e38a95bcd81cc

764C80432619519F750CB0B1A79D1F72C84D032B

Biological materials
Mutant
MGNA-B305 (yjqB::erm), available at the NBRP B. subtilis, Japan
BKE12480 (ΔpghB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGAACGCCCCCCTTT,  downstream forward: _UP4_TAAAACTAGACGGGACCTGC
BKK12480 (ΔpghB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTGAACGCCCCCCTTT,  downstream forward: _UP4_TAAAACTAGACGGGACCTGC
References
Loading

BA7D1D112E934B68073DFC1F76248ED6157240E3

Page visits: 3116

Time of last update: 2025-03-12 19:44:59

Author of last update: Melvin.boenninger