alsS

alsS
168

acetolactate synthase

Locus
BSU_36010
Molecular weight
61.95 kDa
Isoelectric point
5.16
Protein length
571 aaSequenceBlast
Gene length
1713 bpSequenceBlast
Function
overflow metabolism
Product
acetolactate synthase
Essential
no
E.C.
2.2.1.6
Synonyms
alsS
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0028 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,709,628 → 3,711,340
The protein
Catalyzed reaction/ biological activity
H+ + 2 pyruvate --> (2S)-2-acetolactate + CO2 (according to UniProt)
Protein family
TPP enzyme family (according to UniProt)
Cofactors
Mg2 PubMed
TPP PubMed
FAD (according to UniProt)
Structure
4RJJ (PDB) (complex wit TPP) PubMed
Q04789 (AlphaFold)
Paralogous protein(s)
IlvB, YdaP
Expression and Regulation
Operons
Genes
alsS-alsD
Description
PubMed
Regulation
induction by acetate (AlsR) PubMed
expression is heterogeneous PubMed
Regulatory mechanism
AlsR: activation, in the presence of acetate PubMed, in alsR regulon
Rex: repression, if the ratio NADH2/NAD is high PubMed, in rex regulon
stringent response: positive regulation, due to presence of adenines at +1 and +2 positions of the transcript PubMed, in stringent response
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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alsS→alsD

2025-03-16 13:59:18

Jstuelk

88

95ece8b3897d9d4f146bcd915028c1350cc16fc4

AFF8B3E4EA08E038C6FE3D86BD1815CA5F988C58

Biological materials
Mutant
1A979 ( alsS::cat), PubMed, available at BGSC
BKE36010 (ΔalsS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACACCCTCACTCCTTATT,  downstream forward: _UP4_TAGCACTCTGCGCATCACGA
BKK36010 (ΔalsS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACACCCTCACTCCTTATT,  downstream forward: _UP4_TAGCACTCTGCGCATCACGA
LacZ fusion
pGP3327 (cat, based on pAC5]), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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AAA45830B9C5428CADFA5551D33B513E9B1B7C8E

Page visits: 8341

Time of last update: 2025-03-17 01:10:52

Author of last update: Jstuelk