fpa

fpa
168

iron-sensing protein, antirepressor, inhibits the DNA-binding activity of Fur in the absence of Fe2+

Locus
BSU_14840
Molecular weight
10.60 kDa
Isoelectric point
4.61
Protein length
93 aaSequenceBlast
Gene length
282 bpSequenceBlast
Function
control of iron homeostasis
Product
Fur protein antirepressor
Essential
yes
Synonyms
ylaN, furA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4838 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,552,412 → 1,552,693
Phenotypes of a mutant
essential PubMed, the mutant is viable if iron(III) is added to the medium PubMed
The protein
Catalyzed reaction/ biological activity
binds Fe(II) PubMed
binds Fur in the absence of iron to inhibit its DNA-binding activity and to induce genes of the Fur regulon for the Acquisition of iron PubMed
Protein family
UPF0358 family (single member, according to UniProt)
Structure
2ODM (PDB) (from Staphylococcus aureus, 64.6% identity) PubMed
O07638 (AlphaFold)
Expression and Regulation
Operons
Genes
fpa
Description
PubMed
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fpa

2025-03-09 05:25:57

ghost

84

9c386f35d52a9a5b91f355bfd78e7c060acf46ba

86720CC44AA5A190E597707744A4AD4E5A7A2AEA

Biological materials
Mutant
GP3324 (Δfpa::cat), available in Jörg Stülke's lab
GP3321 (Δfur::mls Δfpa::cat), available in Jörg Stülke's lab
BKE14840 (Δfpa::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC,  downstream forward: _UP4_TAAATGATAAAACTCAAACT
BKK14840 (Δfpa::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAAGTATCCCTCCGTTCC,  downstream forward: _UP4_TAAATGATAAAACTCAAACT
Expression vectors
IPTG inducible expression of Strep-ylaN in E. coli: pGP2579 (in pGP172), available in Jörg Stülke's lab
IPTG inducible expression of His-ylaN in E. coli: pGP2583 (in pWH844), available in Jörg Stülke's lab
pGP3897 constitutive overexpression of ylaN, based on pBQ200, available in Jörg Stülke's lab
pGP3900 constitutive expression of Strep-ylaN in B. subtilis, based on pGP380, available in Jörg Stülke's lab
pGP3867 constitutive expression of ylaN-Strep in B. subtilis, based on pGP382, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), pGP3868, pGP3870, pGP3872, pGP3874, available in Jörg Stülke's lab
LacZ fusion
pGP3593 (based on pAC7), available in Jörg Stülke's lab
Antibody
available in Jörg Stülke's lab
References
Reviews
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Original Publications
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Page visits: 5113

Time of last update: 2025-03-14 13:16:31

Author of last update: Jstuelk