ykfC

ykfC
168

D-glutamyl-L-amino acid peptidase

Locus
BSU_12990
Molecular weight
32.91 kDa
Isoelectric point
6.35
Protein length
296 aaSequenceBlast
Gene length
891 bpSequenceBlast
Function
cell wall metabolism
Product
D-glutamyl-L-amino acid peptidase
Essential
no
Synonyms
ykfC
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0791 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,367,941 → 1,368,831
The protein
Catalyzed reaction/ biological activity
cleaves L-Ala-γ-D-Glu dipeptides from cell wall peptides PubMed
The enzyme releases L-Ala-gamma-D-Glu dipeptides from cell wall peptides via cleavage of an L-Ala-gamma-D-Glu-|-L-Lys bond (according to UniProt)
Protein family
Peptidase C40 family (according to UniProt)
Structure
3H41 (PDB) (from B. cereus, 39% identity) PubMed
O35010 (AlphaFold)
Expression and Regulation
Operons
Genes
dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD
Description
PubMed
Regulatory mechanism
CodY: repression, PubMed, in codY regulon
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dppA->ykfD

2025-03-16 16:12:19

Bzhu

139

9978370314fc05087eca13c4f3ce94e92ce93ff2

E2A0CC9BE866DBD9F0E3A5F48EE05465C14EFD9D

Biological materials
Mutant
MGNA-A742 (ykfC::erm), available at the NBRP B. subtilis, Japan
BKE12990 (ΔykfC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGCTGATATGACAGTGT,  downstream forward: _UP4_TTTTCAGAATAAGGAGGGAG
BKK12990 (ΔykfC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGCTGATATGACAGTGT,  downstream forward: _UP4_TTTTCAGAATAAGGAGGGAG
References
Reviews
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Original Publications
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D40ECAE51D9E4791C6FAAA25E2E0D0591D9FAA55

Page visits: 2396

Time of last update: 2025-03-17 00:55:22

Author of last update: Jstuelk