panD

panD
168

L-aspartate-α-decarboxylase

Locus
BSU_22410
Molecular weight
13.76 kDa
Isoelectric point
5.56
Protein length
127 aaSequenceBlast
Gene length
384 bpSequenceBlast
Function
biosynthesis of coenzyme A
Product
L-aspartate-α-decarboxylase
Essential
no
E.C.
4.1.1.11
Synonyms
panD
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0853 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,352,592 → 2,352,975
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
the mutant cells are thinner and shorter than wild type cells PubMed
The protein
Catalyzed reaction/ biological activity
H+ + L-aspartate --> beta-alanine + CO2 PubMed
Protein family
PanD family (single member, according to UniProt)
Structure
2C45 (PDB) (from Mycobacterium tuberculosis, 54% identity) PubMed
P52999 (AlphaFold)
Modification
autocleavage between Gly-24 and Ser-25 to yield the active pyruvyl cofactor that catalyzes decarboxylation PubMed
Expression and Regulation
Operons
Genes
panB-panC-panD-dinG-ypmA-tseB-aspB
Description
PubMed
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panB→aspB

2025-03-10 00:59:56

Jstuelk

73

3C14CF916926052D4B293230C8985ACC791FC72B

3C14CF916926052D4B293230C8985ACC791FC72B

Additional information
formed as an inactive pre-protein, autocleavage between Gly-24 and Her-25 yields the active pyruvyl cofactor that catalyzes decarboxylation PubMed
Biological materials
Mutant
BKE22410 (ΔpanD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCGCTCATCATTGTTCGAT,  downstream forward: _UP4_TAGAAGAAAAGCCCCCTTTA
BKK22410 (ΔpanD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCCGCTCATCATTGTTCGAT,  downstream forward: _UP4_TAGAAGAAAAGCCCCCTTTA
GP3337 (ΔpanD::cat trpC2), available in Jörg Stülke's lab
GP3382 (ΔpanD::neo trpC2), available in Jörg Stülke's lab PubMed
Expression vectors
pGP3725: expression of panD (gapA RBS) by pBQ200 in B. subtilis, available in Jörg Stülke's lab
References
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EE712C1EC9E9C371847C83F7D75C189B276CD4B6

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Time of last update: 2025-03-13 13:38:39

Author of last update: Jstuelk