spsL

spsL
168

dTDP -4-dehydrorhamnose-3,5-epimerase

Locus
BSU_37810
Molecular weight
17.54 kDa
Isoelectric point
4.88
Protein length
151 aaSequenceBlast
Gene length
456 bpSequenceBlast
Function
rhamnose biosynthesis, spore crust polysaccharide synthesis
Product
dTDP -4-dehydrorhamnose-3,5-epimerase
Essential
no
E.C.
5.1.3.13
Synonyms
spsL
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1898 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,882,979 → 3,883,434
Phenotypes of a mutant
production of spores lacking a visible crust PubMed
The protein
Structure
3RYK (PDB) (dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC) from Bacillus anthracis str. Ames with TDP and PPi bound, 32% identity, 56% similarity)
Q7WY56 (AlphaFold)
Expression and Regulation
Operons
Genes
spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL
Description
PubMed
Regulation
expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed
Regulatory mechanism
GerE: activation, PubMed, in gerE regulon
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
SigK: sigma factor, PubMed, in sigK regulon
Additional information
the mRNA is very stable (half-life > 15 min) PubMed
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spsA→spsL

2025-03-14 01:13:46

ghost

133

8fd40e70daa9bc3b7d469fa252e122a98e4555de

9FCD794B68194823BCA66D7E89CC93E5AF9CC0AA

Biological materials
Mutant
BKE37810 (ΔspsL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAATTTTTTCACCTTGA,  downstream forward: _UP4_TAAAAATAGGAACGTGATCA
BKK37810 (ΔspsL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAATTTTTTCACCTTGA,  downstream forward: _UP4_TAAAAATAGGAACGTGATCA
References
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Time of last update: 2025-03-15 02:20:04

Author of last update: Jstuelk