rnhB

rnhB
168

RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication

Locus
BSU_16060
Molecular weight
28.20 kDa
Isoelectric point
5.52
Protein length
255 aaSequenceBlast
Gene length
768 bpSequenceBlast
Function
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
Product
RNase HII
Essential
no
E.C.
3.1.26.4
Synonyms
rnhB, rnh
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0164 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,677,451 → 1,678,218
Phenotypes of a mutant
increased mutation rate PubMed, strand- and sequence-context–dependent GC → AT transitions PubMed
a ''rnhB rnhC'' double mutant grows poorly PubMed
a ''rnhB mutS-mutL'' mutant is not viable PubMed
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
incises DNA-RNA duplexes at aingle rNMPs PubMed
removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication  PubMed
Protein family
RNase HII family (with RnhC, according to UniProt)
Cofactors
Mg2+, Mn2+ PubMed
Structure
2ETJ (PDB) (from Thermotoga maritima, corresponding to aa 69 ... 250, 47% identity)
O31744 (AlphaFold)
Localization
recruited to the replication forks PubMed
Expression and Regulation
Operons
Genes
rnhB-ylqG-ylqH
Description
PubMed
Sigma factors
SigD: sigma factor, PubMed, in sigD regulon
Open in new tab

rnhB→ylqH

2025-03-14 14:28:59

ghost

113

98f7ebb4fcf1623da9e58287f3624156459fba8c

1CC90F1BF46AECEE0BD7591672B817F5E16299E6

Genes
rbgA-rnhB-ylqG-ylqH
Description
PubMed
Open in new tab

rbgA→ylqH

2025-03-09 15:50:12

Jstuelk

110

eeb1b6a4de31a5049ef87638911db24d52f44a7c

3D86AE4ED34D50165869C1951375B5B0E636471C

Biological materials
Mutant
BP423 (ΔrnhB::aphA3) available in Fabian Commichau's lab
BKE16060 (ΔrnhB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC,  downstream forward: _UP4_TAAATCACCATGGACAAGGA
BKK16060 (ΔrnhB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTTTCTCTTCTCCCTTAC,  downstream forward: _UP4_TAAATCACCATGGACAAGGA
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, based on pGP380, expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
References
Reviews
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Original Publications
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D5275ECDC2A70FBAC2CA5980F04B272C3328FC90

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Time of last update: 2025-03-17 03:21:32

Author of last update: Jstuelk