yokI

yokI
168

LXG toxin, non-specific metal-dependent DNase

Locus
BSU_21580
Molecular weight
64.12 kDa
Isoelectric point
5.76
Protein length
571 aaSequenceBlast
Gene length
1716 bpSequenceBlast
Function
competetion with other bacteria in biofilms
Product
LXG toxin, DNase
Essential
no
E.C.
null
Synonyms
yokI
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)

This gene is a member of the following regulons

AbrB regulon

Gene
Coordinates
2,275,706  2,277,421
Phenotypes of a mutant
the mutant is outcompeted by wild type cells in biofilms PubMed
The protein
Domains
LXG domain (aa 1-235) (according to UniProt)
Structure
O31998 (AlphaFold)
Localization
secreted
secretion and delivery requires WXG100 and the T7SS (YukD-EssB-YukB-YueB-YueC-YueD) PubMed
Expression and Regulation
Operons
Genes
yokI-yokJ-yokK-yokL
Description
PubMed
Regulatory mechanism
AbrB: repression, PubMed, in abrB regulon
Additional information
A ncRNA is predicted between 'yolA' and 'yokL' PubMed
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yokI→yokL

2025-03-14 02:07:27

ghost

115

c0991145616d1ecd9ca254c77d6417772671b6cd

0031AF5FF8A7ECE1B60F8B6A925BF05711BF39E6

Biological materials
Mutant
BKE21580 (yokI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCATTCTCCTTTCAAG,  downstream forward: _UP4_AACTTTAGAAAGTAGGTGCG
BKK21580 (yokI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCATTCTCCTTTCAAG,  downstream forward: _UP4_AACTTTAGAAAGTAGGTGCG
References
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13C081250872E72C61C49D8F77AC5AF4B6D431A3

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Time of last update: 2025-03-15 00:08:00

Author of last update: Jstuelk