rnhB
168
RNase HII, endoribonuclease, responsible for removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication
Locus
BSU_16060
Molecular weight
28.20 kDa
Isoelectric point
5.52
Function
endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
Product
RNase HII
Essential
no
E.C.
3.1.26.4
Synonyms
rnhB, rnh
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0164 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,677,451 → 1,678,218
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
Endonucleolytic cleavage to 5'-phosphomonoester (according to UniProt)
incises DNA-RNA duplexes at aingle rNMPs PubMed
removal of single rNMPs incorporated into DNA by DNA polymerase during DNA replication PubMed
Protein family
Mg2+, Mn2+ PubMed
Structure
2ETJ (PDB) (from Thermotoga maritima, corresponding to aa 69 ... 250, 47% identity)
recruited to the replication forks PubMed
Expression and Regulation
Operons
Biological materials
Mutant
BP423 (ΔrnhB::aphA3) available in Fabian Commichau's lab
Expression vectors
for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, based on pGP380, expression from plasmid: pBP500 (E. coli amp & B. subtilis E/L) , available in Fabian Commichau's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
References
Reviews
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Original Publications
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Time of last update: 2025-03-17 01:41:53
Author of last update: Jstuelk