opuAA

opuAA
168

glycine betaine and arsenobetaine ABC transporter (ATP-binding protein)

Locus
BSU_02980
Molecular weight
46.28 kDa
Isoelectric point
5.11
Protein length
418 aaSequenceBlast
Gene length
1257 bpSequenceBlast
Function
compatible solute transport
Product
glycine betaine and arsenobetaine ABC transporter (ATP-binding protein)
Essential
no
E.C.
3.6.3.32
Synonyms
opuAA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4175 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
321,013 → 322,269
The protein
Catalyzed reaction/ biological activity
uptake of glycine betaine and arsenobetaine PubMed
uptake of dimethylglycine PubMed
ATP + H2O + quaternary ammonium --> ADP + H+ + phosphate + quaternary ammonium (according to UniProt)
Protein family
ABC transporter superfamily (according to UniProt)
Domains
2 CBS domains (aa 284-340, aa 344-403) (according to UniProt)
ABC transporter domain (aa 34-270) (according to UniProt)
Structure
3DHW (PDB) (MetN from E. coli, corresponds to aa 10 ... 272, 36% identity) PubMed
P46920 (AlphaFold)
Paralogous protein(s)
OpuBA, OpuCA
Localization
associated to the membrane (via OpuAB) PubMed
Expression and Regulation
Operons
Genes
opuAA-opuAB-opuAC
Description
PubMed
Regulation
induced by osmotic stress PubMed
Regulatory mechanism
RemA: activation, PubMed, in remA regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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opuAA→opuAC

2025-03-09 06:24:28

ghost

113

eafb0a634093d79d1a26ed7a049acac8747a3e05

204CCFDF8D1DFA897A97ACEDDC7D4A3EDDBDB61A

Biological materials
Mutant
GP3618 (spec), available in Jörg Stülke's lab
BKE02980 (ΔopuAA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTTCCCTCCATATTA,  downstream forward: _UP4_CCTTCTGCACAGGAGGTGAA
BKK02980 (ΔopuAA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTTCCCTCCATATTA,  downstream forward: _UP4_CCTTCTGCACAGGAGGTGAA
Expression vectors
pGP2924 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
Labs working on this gene/protein
Erhard Bremer, University of Marburg, Germany homepage
References
Reviews
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Original Publications
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FB2220730013A98D7C979062A4A451518B92DF09

Page visits: 3329

Time of last update: 2025-03-12 12:28:43

Author of last update: Jstuelk